Background: Alcohol use and smoking have been shown to be substantial risk factors for oral cancer, a serious worldwide health problem. The growth of oral cancer cells is thought to be influenced by certain lifestyle choices. Materials and Methods: Oral squamous cell carcinoma, or SCC-25, cell lines were used in a carefully monitored Results: Exposure to ethanol and CSE dramatically decreased cell viability in a way that was dependent on both time and dosage. After 10 μg/mL, 20 μg/mL, and 50 μg/mL, the smoking group's proliferation reduced by 25%, 35%, and 50% after 72 hours, respectively. At 1%, 5%, and 10% ethanol concentrations, the alcohol group also showed reductions of 20%, 30%, and 45%, respectively. With a 60% decrease in proliferation, the combined exposure group showed the strongest inhibition, suggesting a synergistic impact. Statistical significance was noted for every experimental group ( Conclusion: Both alcohol exposure and smoking significantly reduce the growth of oral cancer cells, and their combined effects are synergistic. These results highlight how important lifestyle changes are for managing and preventing oral cancer.