Evidence map›Paper›PMID 40655420›Full record

ArticleBio-protocol2025

Kinase Mobility Shift Assay (KiMSA) for Assessing Protein Kinase A Activity.

Analia G Novero, Tomás J Steeman, Catalina Curcio, Lara Buccolini, Andres Binolfi, Diego Krapf, Mariano G Buffone, Dario Krapf, Cintia Stival

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Analia G NoveroCell Signal Transduction Networks Laboratory, IBR (CONICET-UNR), Rosario, Argentina.
Tomás J SteemanCell Signal Transduction Networks Laboratory, IBR (CONICET-UNR), Rosario, Argentina.
Catalina CurcioCell Signal Transduction Networks Laboratory, IBR (CONICET-UNR), Rosario, Argentina.
Lara BuccoliniCell Signal Transduction Networks Laboratory, IBR (CONICET-UNR), Rosario, Argentina.
Andres BinolfiCellular-Structural Biology Laboratory, IBR (CONICET-UNR), Rosario, Argentina.
Diego KrapfDepartment of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, USA.
Mariano G BuffoneInstituto de Biología y Medicina Experimental (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Dario KrapfCell Signal Transduction Networks Laboratory, IBR (CONICET-UNR), Rosario, Argentina.
Cintia StivalCell Signal Transduction Networks Laboratory, IBR (CONICET-UNR), Rosario, Argentina.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cAMP-dependent protein kinase (PKA) is one of the most extensively distributed kinases among intracellular signal cascades, with a pivotal role in the regulation of various processes, including the capacitation of sperm cells. Traditional assessments of PKA activity rely on the utilization of [γ-

Indexed as

cAMPFertilityFluorescence assayGel electrophoresisKemptide kinase activityKinase assayNon-radioactive assayPhosphorylationProtein kinase A (PKA)Sperm capacitation

Identifiers

PMID40655420
PMCPMC12245630

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.