Evidence map›Paper›PMID 40654943›Full record

ArticlebioRxiv : the preprint server for biology2025

Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells.

Christine M Qabar, Lucas Waldburger, Jay D Keasling, Dan A Portnoy, Jeffery S Cox

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Christine M QabarDepartment of Plant and Microbial Biology, University of California Berkeley, Berkeley CA 94720, USA.ORCID 0000-0002-8345-9225
Lucas WaldburgerDepartment of Bioengineering, University of California Berkeley, Berkeley CA 94720, USA.ORCID 0000-0002-4735-5285
Jay D KeaslingDepartment of Bioengineering, University of California Berkeley, Berkeley CA 94720, USA.ORCID 0000-0003-4170-6088
Dan A PortnoyDepartment of Plant and Microbial Biology, University of California Berkeley, Berkeley CA 94720, USA.ORCID 0000-0003-1218-2799
Jeffery S CoxDepartment of Molecular and Cellular Biology, University of California Berkeley, Berkeley CA 94720, USA.ORCID 0000-0002-5061-6618

Funding

The role of Listeria cyclic-di-AMP during infection and immunityP01AI063302 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI STANLEY, SARAH A · 2004 to 2025
$45.5M
Systems Biology, Bioinformatics, & Data IntegrationU19AI162583 · NIAID · UNIVERSITY OF WASHINGTON · PI HAWN, THOMAS R · 2021 to 2025
$13.0M
Listeria Hemolysis and Escape From a VacuoleR01AI027655 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI DANIEL A PORTNOY · 1993 to 2026
$10.1M
Genetic Dissection of Cells and Organisms Training ProgramT32GM132022 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Nicole King, NOAH K WHITEMAN · 2019 to 2026
$5.2M
NIAID NIH HHS P01 AI063302NIAID NIH HHS R01 AI027655NIAID NIH HHS U19 AI162583NIGMS NIH HHS T32 GM132022
6 · The paper itself

Abstract

There is an urgent need to develop a more efficacious anti-tuberculosis vaccine as the current live-attenuated vaccine strain BCG fails to prevent pulmonary infection in adults. Our long-term goal is to test whether increasing the immunogenicity of BCG will improve vaccine effectiveness while maintaining its proven safety profile. In this study, we leverage a synthetic biology approach to engineer BCG to produce more (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), a phosphoantigen produced as an intermediate of bacterial-but not host-isoprenoid biosynthesis via the methylerythritol phosphate (MEP) pathway. Importantly, HMBPP strongly activates and expands Vγ9Vδ2 T cells, which are unique to higher-order primates and protect against

Identifiers

PMID40654943
PMCPMC12247912

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.