Evidence map›Paper›PMID 40654762›Full record

ArticlebioRxiv : the preprint server for biology2025

Efficient packaging of HIV-1 genomes via recognition of its adenosine-rich content by a heterologous RNA-binding domain.

Hung R Vuong, Qianzi Zhou, Sydney Lesko, Kasyap Tenneti, Keanu Davis, Shanyqua Scott, Daphne Boodwa-Ko, Jenna E Eschbach, Kamya Gopal, Jessica M Porter and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Hung R VuongDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Qianzi ZhouDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Sydney LeskoMcArdle Laboratory for Cancer Research and Institute for Molecular Virology, University of Wisconsin, Madison, USA.
Kasyap TennetiDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Keanu DavisDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Shanyqua ScottMcArdle Laboratory for Cancer Research and Institute for Molecular Virology, University of Wisconsin, Madison, USA.
Daphne Boodwa-KoDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Jenna E EschbachDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Kamya GopalCell and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI USA.
Jessica M PorterDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Yiqing WangDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Shawn MohammedDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Nakyung LeeDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.
Alice TelesnitskyCell and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI USA.ORCID 0000-0001-9878-5931
Nathan ShererMcArdle Laboratory for Cancer Research and Institute for Molecular Virology, University of Wisconsin, Madison, USA.ORCID 0000-0001-9974-236X
Sebla B KutluayDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA.ORCID 0000-0001-5549-7032

Funding

Center for Structural Biology of HIV RNAU54AI170660 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALICE TELESNITSKY · 2022 to 2026
$32.1M
Infectious Diseases/Basic Microbial Pathogenic MechanismsT32AI007172 · NIAID · WASHINGTON UNIVERSITY · PI Daniel E. Goldberg, JENNIFER A PHILIPS · 1985 to 2026
$13.0M
Selective packaging and protection of HIV-1 genomic RNAsR01AI179691 · NIAID · WASHINGTON UNIVERSITY · PI Sebla B. Kutluay · 2024 to 2026
$2.2M
The Cell Biology of HIV-1 Genome TraffickingR56AI110221 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI SHERER, NATHAN M · 2024 to 2024
$457k
NIAID NIH HHS R01 AI179691NIAID NIH HHS R56 AI110221NIAID NIH HHS T32 AI007172NIAID NIH HHS U54 AI170660
6 · The paper itself

Abstract

Selective packaging of a dimeric HIV-1 genome (gRNA) is thought to be driven by specific binding of the nucleocapsid (NC) domain of viral Gag protein to the packaging signal (Ψ) in the host cell cytosol. Through replacement of NC with heterologous RNA-binding domains (RBDs) with distinct RNA-binding properties, we show that the biased adenosine-rich nucleotide content of the gRNA facilitates its selective packaging. Despite disparate RNA binding specificities, all Gag-RBD chimeras successfully recruited the gRNA to the plasma membrane, but many were arrested at the assembly stage. Only the Gag-SRSF5 chimera, which multimerized efficiently on adenosine-rich sequences on the gRNA, packaged gRNA at near wild-type levels. Many Gag chimeras displayed potent dominant negative activities, highlighting NC functions as a targetable step in virus replication. Together, our findings reveal an unexpected aspect of the HIV-1 gRNA, its biased nucleotide content, as a key driver of selective genome packaging.

Identifiers

PMID40654762
PMCPMC12247898

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.