Evidence map›Paper›PMID 40654629›Full record

ArticlebioRxiv : the preprint server for biology2025

Evaluation of Nanopore direct RNA sequencing updates for modification detection.

Neda Ghohabi Esfahani, Andrew J Stein, Stuart Akeson, Talia Tzadikario, Miten Jain

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Epitranscriptomic Analysis of A-to-I RNA Editing and mInternational journal of molecular sciences · 2026
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Neda Ghohabi EsfahaniDepartment of Bioengineering, Northeastern University, Boston, MA, USA.ORCID 0000-0003-0539-4603
Andrew J SteinDepartment of Bioengineering, Northeastern University, Boston, MA, USA.ORCID 0009-0001-6525-6512
Stuart AkesonDepartment of Bioengineering, Northeastern University, Boston, MA, USA.ORCID 0009-0008-8008-2486
Talia TzadikarioDepartment of Bioengineering, Northeastern University, Boston, MA, USA.ORCID 0009-0000-6795-0340
Miten JainDepartment of Bioengineering, Northeastern University, Boston, MA, USA.ORCID 0000-0002-4571-3982

Funding

MFB: Integrating LC-MS/MS and Nanopore Sequencing Platforms to Enable the Direct and Quantatative Detection of RNA ModificationsR01HG013876 · NHGRI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Kristin S Koutmou · 2024 to 2026
$1.2M
NHGRI NIH HHS R01 HG013876
6 · The paper itself

Abstract

Nanopore technology can directly sequence intact RNA molecules, offering a unique capability to read native modifications. Oxford Nanopore Technologies recently updated its direct RNA sequencing technology from RNA002 to RNA004 chemistry. This update included an improved basecaller (Dorado) for increased sequencing accuracy, and ionic current models for

Indexed as

Direct RNA SequencingModification Calling PerformanceNanopore SequencingRNA Modification

Identifiers

PMID40654629
PMCPMC12247956

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.