ArticleJournal of dental sciences2025
The investigation of effects, signal pathways, and applications of high glucose on dental pulp stem cells.
Article in Journal of dental sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background/purpose: Dental pulp stem cells (DPSCs) are among the most widely used dental-derived mesenchymal stem cells (MSCs), and their applications have involved various regions. The glucose metabolism plays a key role in cell function and the current literature presents conflicting evidence regarding the influence of glucose on MSCs' properties. This study evaluated the impact of high glucose (HG) on DPSCs. Materials and methods: DPSCs were stimulated with indicated concentrations of glucose. Cell viability was assessed using a cell counting kit, while apoptosis and autophagy were analyzed via western blot. MSCs immunophenotypic properties were determined by flow cytometry. Osteogenic, adipogenic, and neurogenic differentiation potential were evaluated using western blot, Alizarin red staining, oil red-O staining, and morphological analysis. Results: HG exposure led to a significant decrease in cell viability, with increased apoptosis and autophagy, as indicated by increased levels of cleaved caspase-3, cleaved poly (ADP-ribose) polymerase (PARP), and an elevated microtubule-associated protein 1 light chain 3 beta (LC3B)-II/LC3B-I ratio. However, the immunophenotypic characteristics of DPSCs remained unchanged. DPSCs also demonstrated enhanced osteogenic, adipogenic, and neurogenic differentiation potential by expressing Alizarin red and oil red-O staining, neural-like cell morphology, and several differentiation-related proteins after HG culture stimulation. Conclusion: The present study demonstrated that while HG slightly impairs DPSC viability, it promotes osteogenic, adipogenic, and neurogenic differentiation. Providing valuable insights into the mechanisms by which HG influences various differentiation pathways in DPSCs and establishes a foundation for potential clinical applications of DPSCs in regenerative medicine for diabetic patients.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.