ArticleJournal of dental sciences2025
Beta-glucan promotes dental pulp healing by enhancing cell proliferation, migration, and mineralization.
Article in Journal of dental sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background/purpose: Effective dental pulp healing is essential for preserving tooth vitality. Although beta-glucan has shown promise in wound healing in the medical fields, its potential effects on human dental pulp cells (HDPCs) remain unexplored. This study aimed to assess beta-glucan's effects on HDPC proliferation, migration, collagen synthesis, mineralization, and differentiation. Materials and methods: Primary HDPCs were cultured and assigned into five groups: control, vehicle, and beta-glucan at concentrations of 5, 7.5, and 10 mg/mL. Cell proliferation was quantified using the alamarBlue® assay at 24, 48, and 72 h. Cell migration was assessed at 12 and 24 h via the scratch wound healing assay. Flow cytometry was employed to detect integrin beta 1 (CD29) expression during wound healing. Mineralization and differentiation at day 14 were evaluated through alizarin red S staining and quantitative real-time polymerase chain reaction (qRT-PCR), measuring Dentin Sialophosphoprotein (DSPP), Interleukin-10 (IL-10), and Collagen type I (COL1) gene expression. Statistical significance was established at Results: At 24 and 72 h, all concentrations of beta-glucan significantly induced cell proliferation. In the wound healing assay, beta-glucan improved cell migration and increased the expression of integrin beta 1 after 24 h. Mineralized matrix formation and the expression of IL-10 and COL1 were significantly observed at 14 days. The upregulation of DSPP was detected in groups supplemented with 5 and 7.5 mg/mL beta-glucan. Conclusion: Beta-glucan enhanced cell proliferation, cell migration potential, integrin beta 1 expression, mineralized matrix formation, and DSPP, IL-10, and COL1 gene expression in HDPCs.
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