ArticleCirculation2025
DNA Methylation Signatures of Cardiovascular Health Provide Insights Into Diseases.
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Article in Circulation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of Genetics in Stroke Risk and Outcome: A Review of Current Evidence.Brain and behavior · 2025Pooled it
- Proteomic Mendelian randomization and machine learning reveal causal plasma biomarkers in cardiorenal comorbidity.Renal failure · 2026Article
- Sex-dependent epigenomic and transcriptomic reprogramming links maternal obesity to cardiac remodeling in adult offspring.American journal of physiology. Heart and circulatory physiology · 2026Article
- Blood-Based DNA Methylation Models Improve Short-term Cardiovascular Risk Stratification in Individuals With Type 2 Diabetes.Diabetes care · 2026Article
- Epigenetic regulation in atherosclerosis and its therapeutic potential.Nature reviews. Cardiology · 2026Review
- Longitudinal Blood DNA Methylation Changes During Weight-Loss Intervention and Dementia Progression Risk.Research square · 2026Article
- Paternal sleep deprivation and cardiac dysfunction in offspring: A perspective of myocardial hypertrophy.Experimental physiology · 2026Article
- DNA Methylation: A Key Epigenetic Regulator of Cardiovascular Diseases.Reviews in cardiovascular medicine · 2026Review
- DNA methylation signatures of Life's Essential 8 and their implications for dementia.Alzheimer's research & therapy · 2025Article
- Epigenetic crossroads in metabolic and cardiovascular health: the role of DNA methylation in type 2 diabetes and cardiovascular diseases.Cardiovascular diabetology · 2025Review
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Authors and funding
48 authors.
Madeleine CarbonneauPopulation Sciences Branch, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD (M.C., T.H., R.J., D.L.).ORCID 0009-0002-4102-5952
Yi LiDepartment of Biostatistics, Boston University School of Public Health, Boston, MA (Y. Li, M.W., C.L., V.X.).ORCID 0000-0002-5544-750X
Yishu QuDepartment of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL (Y.Q., Y.Z., J.W., H.N., L.H., D.L.-J.).ORCID 0000-0001-5937-3811
Yinan ZhengDepartment of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL (Y.Q., Y.Z., J.W., H.N., L.H., D.L.-J.).ORCID 0000-0002-2006-7320
Alexis C WoodUnited States Department of Agriculture/ARS Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX (A.C.W.).ORCID 0000-0001-7616-2119
Mengyao WangDepartment of Biostatistics, Boston University School of Public Health, Boston, MA (Y. Li, M.W., C.L., V.X.).
Chunyu LiuDepartment of Biostatistics, Boston University School of Public Health, Boston, MA (Y. Li, M.W., C.L., V.X.).ORCID 0000-0002-9160-0153
Tianxiao HuanPopulation Sciences Branch, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD (M.C., T.H., R.J., D.L.).
Roby JoehanesPopulation Sciences Branch, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD (M.C., T.H., R.J., D.L.).ORCID 0000-0001-5549-9054
Xiuqing GuoDepartment of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA (X.G., J.Y., K.D.T., J.I.R.).ORCID 0000-0002-5264-5068
Jie YaoDepartment of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA (X.G., J.Y., K.D.T., J.I.R.).ORCID 0000-0002-8822-0651
Kent D TaylorDepartment of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA (X.G., J.Y., K.D.T., J.I.R.).ORCID 0000-0002-2756-4370
Russell P TracyDepartment of Pathology and Laboratory Medicine, University of Vermont Larner College of Medicine, Colchester, VT (R.P.T., P.D.).ORCID 0000-0002-0080-2420
Peter DurdaDepartment of Pathology and Laboratory Medicine, University of Vermont Larner College of Medicine, Colchester, VT (R.P.T., P.D.).ORCID 0000-0003-4000-2943
Yongmei LiuDuke Molecular Physiology Institute, Duke University, Durham, NC (Y. Liu).ORCID 0000-0002-6562-1858
W Craig JohnsonDepartments of Biostatistics (W.C.J.), University of Washington, Seattle, WA.ORCID 0000-0002-3161-3753
Wendy S PostDepartment of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD (W.S.P.).ORCID 0000-0002-8655-5204
Tom BlackwellDepartment of Biostatistics, School of Public Health, University of Michigan, Ann Arbor, MI (T.B.).
Jerome I RotterDepartment of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA (X.G., J.Y., K.D.T., J.I.R.).ORCID 0000-0001-7191-1723
Stephen S RichDepartment of Genome Sciences, University of Virginia School of Medicine, Charlottesville, VA (S.S.R.).ORCID 0000-0003-3872-7793
Susan RedlineDepartments of Medicine and Neurology, Brigham & Women's Hospital and Harvard Medical School, Boston, MA (S.R.).ORCID 0000-0002-6585-1610
Myriam FornageBrown Foundation Institute of Molecular Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX (M.F.).ORCID 0000-0003-0677-8158
Jun WangDepartment of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL (Y.Q., Y.Z., J.W., H.N., L.H., D.L.-J.).
Hongyan NingDepartment of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL (Y.Q., Y.Z., J.W., H.N., L.H., D.L.-J.).ORCID 0000-0003-1157-6614
Lifang HouDepartment of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL (Y.Q., Y.Z., J.W., H.N., L.H., D.L.-J.).ORCID 0000-0003-4877-0031
Donald Lloyd-JonesDepartment of Preventive Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL (Y.Q., Y.Z., J.W., H.N., L.H., D.L.-J.).ORCID 0000-0003-0847-6110
Kendra FerrierDepartment of Bioinformatics and Personalized Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO (K.F.).ORCID 0000-0002-8813-6871
Yuan-I MinDepartment of Medicine, University of Mississippi Medical Center, Jackson, MS (Y.-I.M., A.P.C.).ORCID 0000-0003-2470-0004
April P CarsonDepartment of Medicine, University of Mississippi Medical Center, Jackson, MS (Y.-I.M., A.P.C.).ORCID 0000-0002-7970-6756
Laura M RaffieldDepartment of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC (L.M.R.).ORCID 0000-0002-7892-193X
Alexander TeumerDepartment of Psychiatry and Psychotherapy (A.T., H.J.G.), University Medicine Greifswald, Greifswald, Germany.ORCID 0000-0002-8309-094X
Hans J GrabeDepartment of Psychiatry and Psychotherapy (A.T., H.J.G.), University Medicine Greifswald, Greifswald, Germany.ORCID 0000-0003-3684-4208
Henry VölzkeDepartment SHIP/Clinical-Epidemiological Research, Institute for Community Medicine (H.V.), University Medicine Greifswald, Greifswald, Germany.
Matthias NauckInstitute of Clinical Chemistry and Laboratory Medicine (M.N.), University Medicine Greifswald, Greifswald, Germany.ORCID 0000-0002-6678-7964
Marcus DörrDepartment of Internal Medicine B (M.D.), University Medicine Greifswald, Greifswald, Germany.ORCID 0000-0001-7471-475X
Arce Domingo-RellosoDepartments of Biostatistics (A.D.-R.).ORCID 0000-0001-6928-8290
Amanda FrettsEpidemiology, Cardiovascular Health Research Unit (A.F.), University of Washington, Seattle, WA.ORCID 0000-0002-5358-2345
Maria Tellez-PlazaDepartment of Chronic Disease Epidemiology, National Center for Epidemiology, Instituto de Salud Carlos III, Madrid, Spain (M.T.-P.).ORCID 0000-0002-3850-1228
Shelley A ColePopulation Health Program, Texas Biomedical Research Institute, San Antonio, TX (S.A.C.).ORCID 0000-0002-2651-0127
Ana Navas-AcienEnvironmental Health Sciences (A.D.-R., A.N.-A.).ORCID 0000-0001-9824-7797
Meng WangDepartment of Biostatistics, Boston University School of Public Health, Boston, MA (Y. Li, M.W., C.L., V.X.).ORCID 0000-0001-8345-9180
Joanne M MurabitoFramingham Heart Study, Framingham, MA (M.C., T.H., R.J., J.M.M., N.L.H.-C., V.X., D.L.).ORCID 0000-0002-0192-7516
Nancy L Heard-CostaFramingham Heart Study, Framingham, MA (M.C., T.H., R.J., J.M.M., N.L.H.-C., V.X., D.L.).ORCID 0000-0001-9730-0306
Brenton PrescottSection of Preventive Medicine and Epidemiology, Boston University School of Medicine, Boston, MA (B.P., V.X.).ORCID 0000-0002-3805-9070
Vanessa XanthakisFramingham Heart Study, Framingham, MA (M.C., T.H., R.J., J.M.M., N.L.H.-C., V.X., D.L.).ORCID 0000-0002-7352-621X
Dariush MozaffarianNutrition Epidemiology, and Data Science, Friedman School of Nutrition Science and Policy, Tufts University, Boston, MA (M.W., D.M., J.M.).ORCID 0000-0001-7958-9492
Daniel Levy *Population Sciences Branch, Division of Intramural Research, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD (M.C., T.H., R.J., D.L.).ORCID 0000-0003-1843-8724
Jiantao Ma *Nutrition Epidemiology, and Data Science, Friedman School of Nutrition Science and Policy, Tufts University, Boston, MA (M.W., D.M., J.M.).ORCID 0000-0001-7478-7203
Funding
Large Scale Sequencing and Analysis of GenomesU54HG003067 · NHGRI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GABRIEL, STACEY, LANDER, ERIC S · 2004 to 2015
$568.6MThe Southwest National Primate Research Center Supplement- Infrastructure improvements of ABSL2 holding areasP51OD011133 · OD · TEXAS BIOMEDICAL RESEARCH INSTITUTE · PI Larry S. Schlesinger · 2012 to 2026
$129.7MUCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1MInstitute for Clinical and Translational ResearchUL1TR001079 · NCATS · JOHNS HOPKINS UNIVERSITY · PI FORD, DANIEL ERNEST · 2013 to 2017
$60.1MTrue Metal fac coreP30ES009089 · NIEHS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Steven N. Chillrud · 1998 to 2026
$44.7MTransgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MILES Frome WILKINSON · 2003 to 2026
$40.4MWake Forest Clinical and Translational Science AwardUL1TR001420 · NCATS · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI ARD, JAMY D, FOLEY, KRISTIE L · 2015 to 2023
$32.3MFRAMINGHAM HEART STUDY - YEAR 5 EXAM75N92019D00031 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI RAMACHANDRAN, VASAN · 2019 to 2024
$29.8MClinical and Translational Science AwardUL1TR000040 · NCATS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI GINSBERG, HENRY N · 2012 to 2015
$26.2MThe Strong Heart Study, Phase VU01HL041654 · NHLBI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI LEE, ELISA TAN · 1988 to 2013
$19.2MTask Area A Core Study Operations.Task Area A shall encompass annual follow-up of cohort members, clinical endpoints ascertainment, study coordination activities, maintenance of the database and biosp75N92020D00001 · NHLBI · UNIVERSITY OF WASHINGTON · PI MCCLELLAND, ROBYN LEAGH · 2020 to 2025
$17.2MSTRONG HEART STUDY (SHS): OUHSC COORDINATING CENTER - TASK AREA B (B.1 AND B.3)75N92019D00027 · NHLBI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI ZHANG, YING · 2019 to 2025
$15.5MNCATS NIH HHS UL1 TR000040NCATS NIH HHS UL1 TR001079NCATS NIH HHS UL1 TR001420NCATS NIH HHS UL1 TR001881NHGRI NIH HHS U54 HG003067NHLBI NIH HHS 75N92019D00027NHLBI NIH HHS 75N92019D00028NHLBI NIH HHS 75N92019D00029NHLBI NIH HHS 75N92019D00030NHLBI NIH HHS 75N92019D00031NHLBI NIH HHS 75N92020D00001NHLBI NIH HHS 75N92020D00002NHLBI NIH HHS 75N92020D00003NHLBI NIH HHS 75N92020D00004NHLBI NIH HHS 75N92020D00005NHLBI NIH HHS 75N92020D00006NHLBI NIH HHS 75N92020D00007NHLBI NIH HHS 75N92023D00002NHLBI NIH HHS 75N92023D00003NHLBI NIH HHS 75N92023D00004NHLBI NIH HHS 75N92023D00005NHLBI NIH HHS 75N92023D00006NHLBI NIH HHS K22 HL135075NHLBI NIH HHS N01 HC025195NHLBI NIH HHS N01 HC095159NHLBI NIH HHS N01 HC095160NHLBI NIH HHS N01 HC095161NHLBI NIH HHS N01 HC095162NHLBI NIH HHS N01 HC095163NHLBI NIH HHS N01 HC095164NHLBI NIH HHS N01 HC095165NHLBI NIH HHS N01 HC095166NHLBI NIH HHS N01 HC095167NHLBI NIH HHS N01 HC095168NHLBI NIH HHS N01 HC095169NHLBI NIH HHS N02 HL064278NHLBI NIH HHS R01 HL090863NHLBI NIH HHS R01 HL105756NHLBI NIH HHS R01 HL109282NHLBI NIH HHS R01 HL109284NHLBI NIH HHS R01 HL109301NHLBI NIH HHS R01 HL109315NHLBI NIH HHS R01 HL109319NHLBI NIH HHS R01 HL117626NHLBI NIH HHS R01 HL120393NHLBI NIH HHS R01 HL155569NHLBI NIH HHS U01 HL041642NHLBI NIH HHS U01 HL041652NHLBI NIH HHS U01 HL041654NHLBI NIH HHS U01 HL065520NHLBI NIH HHS U01 HL065521NHLBI NIH HHS U01 HL120393NIAAA NIH HHS R01 AA028263NIDDK NIH HHS P30 DK063491NIEHS NIH HHS P30 ES009089NIEHS NIH HHS P42 ES033719NIEHS NIH HHS R01 ES021367NIEHS NIH HHS R01 ES025216NIEHS NIH HHS R01 ES032638NIH HHS P51 OD011133
Abstract
backgroundThe association of overall cardiovascular health (CVH) with changes in DNA methylation (DNAm) has not been well characterized.
methodsWe calculated the American Heart Association's Life's Essential 8 score to reflect CVH in 5 cohorts with diverse backgrounds (mean age 54 years, 55% women, and enrollment year ranging from 1989 to 2012). Epigenome-wide association studies (EWAS) for Life's Essential 8 score were conducted, followed by bioinformatic analyses. DNAm loci significantly associated with Life's Essential 8 score were used to calculate a CVH DNAm score. We examined the association of the CVH DNAm score with incident cardiovascular disease (CVD), cardiovascular disease-specific mortality, and all-cause mortality.
resultsWe identified 609 cytosine-phosphate-guanines (CpGs) associated with Life's Essential 8 score at false discovery rate<0.05 in the discovery analysis and at Bonferroni-corrected
conclusionsWe identified new DNAm signatures for CVH across diverse cohorts. Our analyses indicate that multiple biological pathways may be relevant to the observed association between CVH and clinical outcomes.
Indexed as
Cardiovascular DiseasesDNA MethylationEpigenesis, GeneticAdultAgedCpG IslandsFemaleGenome-Wide Association StudyHumansMaleMiddle Agedall-cause mortalitycardiovascular diseasescardiovascular healthDNA methylationepigenome-wide association studyLife’s Essential 8
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