Evidence map›Paper›PMID 40653863›Full record

ArticlePlant, cell & environment2025

Argonaute1-Dependent LtmilR2 Negatively Regulated Infection of Lasiodiplodia theobromae by Targeting a Guanine Nucleotide Exchange Factor in RAS Signalling.

Caiping Huang, Yuanyuan He, Wei Zhang, Jingpeng Wang, Mei Liu, Borala Liyanage D Deepali, Jiye Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant, cell & environment, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Caiping HuangBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.ORCID 0000-0002-1359-0412
Yuanyuan HeBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Wei ZhangBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Jingpeng WangBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Mei LiuBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Borala Liyanage D DeepaliBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Jiye YanBeijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Funding

This study has received funding from the Outstanding Scientist Project of Beijing Academy of Agriculture and Forestry Sciences (JKTD2025002), National Natural Science Foundation grant 32402301, Young Scientist Project of Beijing Academy of Agriculture and Forestry Sciences grant QNJJ202425 and National Technology System for Grape Industry CARS-29.
6 · The paper itself

Abstract

Lasiodiplodia theobromae (L. theobromae) is the causative agent of grapevine canker disease, which is a serious threat to global grape production. Currently, no effective fungicides are available to manage this disease. In this study, LtmilR2 was identified as an Argonaute1 (AGO1)-dependent small RNA produced by L. theobromae. Functional analysis revealed that both LtAGO1 and LtmilR2 negatively regulated the pathogenicity of L. theobromae. Through degradome sequencing, we identified LtRASGEF-a guanine nucleotide exchange factor involved in RAS signalling-as the target gene of LtmilR2. LtmilR2 suppressed the expression of LtRASGEF. Pathogenicity assays demonstrated that LtRASGEF was essential for fungal virulence. Furthermore, following infection, the expression of LtmilR2 rapidly decreased, while LtRASGEF expression quickly increased and positively regulated infection. Finally, we observed that the growth of L. theobromae was inhibited when an RNA duplex targeting LtmilR2 was delivered via the star polycation (SPc) nanocarrier, suggesting that LtmilR2 may serve as an RNA-based fungicide target for grapevine canker disease management.

Indexed as

Argonaute ProteinsAscomycotaFungal ProteinsPlant Diseasesras Guanine Nucleotide Exchange FactorsVitisGene Expression Regulation, FungalMicroRNAsVirulenceArgonaute ProteinsFungal ProteinsMicroRNAsras Guanine Nucleotide Exchange Factorsgrapevine canker diseaseLasiodiplodia theobromaemilRNApathogenicityRNA fungicide

Identifiers

PMID40653863
PMCPMC12415416

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.