Evidence map›Paper›PMID 40653746›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.

Enbo Zhu, Yaran Zhang, Peng Zhao, Jae Min Cho, Zhaoqiang Wang, Yan-Ruide Li, Jing Wang, Samuel Margolis, Shaolei Wang, Lili Yang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Enbo ZhuDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.ORCID https://orcid.org/0000-0001-8600-8553
Yaran ZhangDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Peng ZhaoDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Jae Min ChoDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Zhaoqiang WangDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Yan-Ruide LiDepartment of Microbiology, Immunology & Molecular Genetics, UCLA, Los Angeles, CA, 90095, USA.
Jing WangDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Samuel MargolisDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA.
Shaolei WangDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Lili YangDepartment of Microbiology, Immunology & Molecular Genetics, UCLA, Los Angeles, CA, 90095, USA.
Alison ChuDivision of Neonatology and Developmental Biology, Department of Pediatrics, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA.
Yuhua ZhangDoheny Eye Institute, Department of Ophthalmology, UCLA, Los Angeles, CA, 91103, USA.
Liang GaoDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.
Tzung K HsiaiDepartment of Bioengineering, UCLA, Los Angeles, CA, 90095, USA.ORCID https://orcid.org/0000-0003-1734-0792

Funding

Shear Stress and Light-Sheets to Study Cardiac TrabeculationR01HL129727 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2015 to 2024
$3.5M
In vivo Ultrastructure of Chorioretinal DiseaseR01EY024378 · NEI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ZHANG, YUHUA LIANG · 2015 to 2023
$2.9M
Intravascular Deployment of a Wirelessly Powered Micro-PacerR01HL149808 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Tzung K Hsiai · 2020 to 2026
$2.8M
Kilohertz 3D Optical Mapping of Atrial Fibrillation in Beating Zebrafish HeartsR01HL165318 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, HSIAI, TZUNG K · 2022 to 2025
$2.2M
Integrating Volumetric Light-Field with Computational Fluid Dynamics to Study Myocardial Trabeculation and FunctionR01HL159970 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, MARSDEN, ALISON L · 2021 to 2024
$2.0M
Targeting epithelial membrane protein 2 (EMP2) in retinopathy of prematurityR01EY032561 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHU, ALISON · 2021 to 2025
$2.0M
In Vivo Characterizations of Retinal HemodynamicsR01EY034218 · NEI · DOHENY EYE INSTITUTE · PI ZHANG, YUHUA LIANG · 2022 to 2025
$1.5M
UCLA and Caltech integrated Cardiovascular Medicine for Bioengineers (iCMB)T32HL144449 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HSIAI, TZUNG K, PAJUKANTA, PAIVI · 2020 to 2024
$1.2M
NEI NIH HHS R01 EY024378NEI NIH HHS R01EY024378NEI NIH HHS R01 EY032561NEI NIH HHS R01EY032561NEI NIH HHS R01 EY034218NEI NIH HHS R01EY034218NHLBI NIH HHS R01 HL129727NHLBI NIH HHS R01HL129727NHLBI NIH HHS R01 HL149808NHLBI NIH HHS R01 HL159970NHLBI NIH HHS R01HL159970NHLBI NIH HHS R01 HL165318NHLBI NIH HHS R01HL165318NHLBI NIH HHS T32 HL144449NHLBI NIH HHS T32HL144449
6 · The paper itself

Abstract

Light-sheet fluorescence microscopy (LSFM) enables rapid data acquisition with minimal phototoxicity, while optical clearing reduces light scattering by matching sample and imaging medium refractive indices (RIs). Emerging clearing methods extend from cells and organoids to entire organs and organisms, prompting macro-view LSFM microscopes with macro-objectives for low-magnification imaging of larger specimens at adequate resolution. In cardiovascular studies, multiple organs often require imaging, yet clearing protocols optimized for different organs alter the sample RIs inconsistently. Standard-size dipping objectives use correction collars for RI adaptation, but macro-objectives, owing to their large size and long working distances, are typically dry lenses lacking built-in RI correction. An RI-corrected (rc)-LSFM macro-view system addresses this limitation by accommodating a broad range of RI values. By integrating axial sweeping, multi-view imaging, and a closed quartz chamber, the rc-LSFM improves field of view (up to ≈8.8 mm), isotropy, spatial resolution (≈3 µm), and operational safety. It effectively visualizes microvascular networks in zebrafish embryos and post-natal mouse retina, traces the stem cell lineage of cardiomyocytes in mouse embryos, and reveals sympathetic nerve innervation in adult mouse aorta. The rc-LSFM macro-view system is compatible with various optical clearing protocols for multi-scale imaging with high isotropy and spatial resolution.

Indexed as

Cardiovascular SystemRefractometryAnimalsMiceMicroscopy, FluorescenceZebrafishcardiovascular Imaginglarge field of viewmacro‐view light‐sheet fluorescent microscopyrefractive index correction

Identifiers

PMID40653746
PMCPMC12520573

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.