ArticleBMC oral health2025
Oxidative genomic damage in pediatric patients exposed to mercury released by dental amalgam.
Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMercury (Hg) is the major component of dental amalgam which has been utilized for decades because it is durable, inexpensive and easy to manipulate and position, as well as having a relatively low cost. Hg from dental amalgam fillings has been shown to be released into the buccal cavity due to processes such as abrasion and corrosion, and which could represent a continuous source of oxidative damage to mouth tissues. Therefore, this study assessed the impact of Hg-containing dental amalgams exposure on the oxidative genomic damage using a means of the buccal micronucleus cytome assay by counting the nuclear abnormalities (NAs) in buccal mucosa cells and by analyzing in whole saliva the molecules 8-hydroxy-2′-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) in whole saliva.
methodsThis study was conducted on 120 healthy children divided into two groups-group 1 (or the control group) (n = 60), including participants without dental caries, no history of dental amalgam (Hg-containing) restorations, and no other dental treatments, and group 2 (the intervention group) (n = 60), including participants with dental caries involving enamel or dentin, who received dental amalgam restorations in two posterior teeth. Oral epithelial cell and whole saliva samples were taken at baseline and 30 days later for group 1, while for group 2, samples were obtained at different pre-treatment (baseline) and post-amalgam placement time points at 15 and 30 days.
resultsAn increased frequency of NAs (p < 0.05) and higher levels of 8-OHdG (p < 0.05) and MDA (p < 0.05) were observed after Hg-dental amalgams exposure. In addition, a positive correlation existed between NAs and oxidative stress induced by Hg from dental amalgam.
conclusionsThe presents study shows that individuals exposed to Hg-containing dental amalgam exhibit increased NAs in oral epithelial cells, as well as increased levels of 8-OHdG and MDA in saliva, which are directly related to genotoxicity, oxidative DNA damage and lipid peroxidation, respectively. Exposure to Hg- containing dental amalgams may contribute to oxidative stress and genetic damage, potentially leading to long-term implications for both oral and systemic health.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.