Evidence map›Paper›PMID 40653101›Full record

ArticleChemico-biological interactions2025

Kinetic and structural evidence for specific DMSO interference with reversible binding of uncharged bis-oximes to hAChE and their reactivation kinetics of OP-hAChE.

Dora Kolić, Oksana Gerlits, Megan Kucharski, Lukas Gorecki, Nichole Joiner, Andrey Kovalevsky, Zoran Radić

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemico-biological interactions, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dora KolićInstitute for Medical Research and Occupational Health, HR-10001, Zagreb, Croatia.
Oksana GerlitsDepartment of Natural Sciences, Tennessee Wesleyan University, Athens, TN, 37303, USA.
Megan KucharskiSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, 92093-0650, USA.
Lukas GoreckiDepartment of Toxicology and Military Pharmacy, Military Faculty of Medicine, University of Defence, 500 01, Hradec Kralove, Czech Republic; Biomedical Research Center, University Hospital Hradec Kralove, 500 05 Hradec Kralove, Czech Republic.
Nichole JoinerSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, 92093-0650, USA.
Andrey KovalevskyNeutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.
Zoran RadićSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, 92093-0650, USA. Electronic address: zradic@ucsd.edu.

Funding

Accelerated AChE Reactivator Design by Mechanistic Neutron Scattering StudiesU01NS083451 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RADIC, ZORAN · 2014 to 2018
$3.4M
Refined uncharged Bis-Oximes for Rapid CNS Reactivation of OP-Inhibited hAChE.R21NS120839 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RADIC, ZORAN · 2022 to 2023
$440k
In Vivo Efficacy of Novel Uncharged Bis-Oximes in OP Poisoning TreatmentR21NS120884 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RADIC, ZORAN · 2022 to 2023
$366k
NINDS NIH HHS R21 NS120839NINDS NIH HHS R21 NS120884NINDS NIH HHS U01 NS083451
6 · The paper itself

Abstract

The structural basis of inhibitory effect of organic solvent dimethyl sulfoxide (DMSO) on human acetylcholinesterase (EC 3.1.1.7; hAChE) was inferred from the effect of DMSO on kinetics of reversible inhibition of uncharged, heterocyclic bis-oximes to hAChE, from DMSO effect on rates of reactivation of inactive organophosphate (OP)-hAChE conjugates by bis-oximes and by X-ray structures of bis-oxime and DMSO binding to hAChE. The reversible inhibition constant of DMSO for hAChE in 0.1 M phosphate buffer pH 7.4 at 22 °C, was K

Indexed as

AcetylcholinesteraseDimethyl SulfoxideOximesBinding SitesCholinesterase InhibitorsCrystallography, X-RayHumansKineticsProtein BindingAcetylcholinesteraseCholinesterase InhibitorsDimethyl SulfoxideOximesAcetylcholinesterase inhibitionAcetylcholinesterase reactivationAntidotesDMSOHeterocyclic bis-oximesOrganic solventOrganophosphorus compoundsParaoxonVX

Identifiers

PMID40653101
PMCPMC13513810

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.