ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2025
PeakClimber: A software tool for the accurate quantification of complex HPLC chromatograms.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
High-performance liquid chromatography (HPLC) is a common medium-throughput technique to quantify the components of complex mixtures like those typically obtained from biological tissue extracts. However, analysis of HPLC data from multianalyte samples is hampered by a lack of tools to accurately determine the precise analyte quantities on a level of precision equivalent to mass spectrometry approaches. To address this problem, we developed a tool we call PeakClimber that uses a sum of bidirectional exponentially modified Gaussian (BEMG) functions to accurately deconvolve overlapping, multianalyte peaks in HPLC traces. Here we show that HPLC peaks are well-fit by a BEMG function, that PeakClimber more accurately quantifies known peak areas than standard industry software and other open-source software packages for HPLC, and that PeakClimber accurately quantifies differences in triglyceride abundances between colonized and germ-free fruit flies.
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