Evidence map›Paper›PMID 40652291›Full record

ArticleHereditas2025

Reduced miR-338-3p contributes to polycystic ovarian syndrome by inhibiting proliferation and enhancing apoptosis.

Lin Liang, Jie Lv, Wei Li, Chengwen Song, Ying Chen, Huafang Wei

Abstract read
In one paragraph

Article in Hereditas, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Frontiers in bioengineering and biotechnology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lin Liang *Department of Gynecology, General Hospital of Central Theater Command of Chinese People's Liberation Army, 627 Wuluo Road, Wuchang District, Hubei, Wuhan, 430064, China.
Jie Lv *Department of Gynecology, General Hospital of Central Theater Command of Chinese People's Liberation Army, 627 Wuluo Road, Wuchang District, Hubei, Wuhan, 430064, China.
Wei LiDepartment of Gynecology, General Hospital of Central Theater Command of Chinese People's Liberation Army, 627 Wuluo Road, Wuchang District, Hubei, Wuhan, 430064, China.
Chengwen SongDepartment of Gynecology, General Hospital of Central Theater Command of Chinese People's Liberation Army, 627 Wuluo Road, Wuchang District, Hubei, Wuhan, 430064, China.
Ying ChenDepartment of Gynecology, General Hospital of Central Theater Command of Chinese People's Liberation Army, 627 Wuluo Road, Wuchang District, Hubei, Wuhan, 430064, China.
Huafang WeiDepartment of Gynecology, General Hospital of Central Theater Command of Chinese People's Liberation Army, 627 Wuluo Road, Wuchang District, Hubei, Wuhan, 430064, China. weihuafang05@163.com.

Funding

2024 Hubei Provincial Natural Science Foundation Joint Fund for Innovation and Development Key Project 2024AF0276
6 · The paper itself

Abstract

backgroundPolycystic ovarian syndrome (PCOS) is a frequently occurring disorder affecting reproductive and metabolic health. miR-338-3p is implicated in early follicular development. We aimed to investigate the expression of miR-338-3p in PCOS patients and its effects on proliferation and apoptosis of ovarian granulosa cells.

methodsThe study included 100 healthy women and 110 women diagnosed with PCOS as participants. Reverse transcription quantitative PCR (RT-qPCR) was used to detect the expression of miR-338-3p and phosphatase and tensin homolog (PTEN), and receiver operating characteristic (ROC) was employed to evaluate the diagnostic efficacy of miR-338-3p. Cell proliferation and apoptosis were detected by Cell Counting Kit-8 (CCK-8) and flow cytometry. Pearson correlation analysis was used to assess the correlations between miR-338-3p and luteinizing hormone (LH), testosterone, or PTEN. The target relationship of miR-338-3p and PTEN was confirmed via dual-luciferase assay.

resultsSerum miR-338-3p was decreased in PCOS patients, and it was negatively correlated with both LH and testosterone. Downregulation of miR-338-3p inhibits the proliferation of ovarian granulosa cells and enhances cell apoptosis, whereas upregulation produces the opposite effect. PTEN inhibition subverted the inhibited proliferation and enhanced apoptosis regulated by miR-338-3p inhibitor.

conclusionsReduced miR-338-3p levels have potential predictive value in distinguishing individuals with PCOS patients from normal population. Mechanistically, this microRNA regulates the PTEN gene to inhibit granulosa cell proliferation and promote apoptosis, thereby contributing to the pathological processes of PCOS.

Indexed as

ApoptosisCell ProliferationMicroRNAsPolycystic Ovary SyndromeAdultCase-Control StudiesFemaleGranulosa CellsHumansLuteinizing HormonePTEN PhosphohydrolaseTestosteroneYoung AdultLuteinizing HormoneMicroRNAsMIRN338 microRNA, humanPTEN PhosphohydrolasePTEN protein, humanTestosteroneApoptosismiR-338-3pPolycystic ovarian syndromeProliferation

Identifiers

PMID40652291
PMCPMC12254983

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.