Evidence map›Paper›PMID 40652213›Full record

ArticleBMC genomic data2025

Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda.

Danladi Makeri, Emmanuel Eilu, Martin Odoki, Ismail Abiola Adebayo, Reuben Maghembe, Musoba Abubakar, Reagan Muhwezi, Theophilus Pius, Priscilla Peter Dilli, Saheed Adekunle Akinola and 1 more

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Article in BMC genomic data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Danladi MakeriDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda. makeri@kiu.ac.ug.
Emmanuel EiluDepartment of Microbiology and Immunology, School of Medicine, King Ceasor University, Kampala, Uganda.
Martin OdokiDepartment of Applied Sciences, School of Sciences, Nkumba University, Entebbe, Uganda.
Ismail Abiola AdebayoDepartment of Microbiology, Parasitology, and Immunology, School of Medicine, Kabale University, Kabale, Uganda.
Reuben MaghembeDepartment of Immunology and Molecular Biology, College of Health Sciences, Makerere University, Kampala, Uganda.
Musoba AbubakarInstitute of Biomedical Research, Kampala International University Western Campus, Ishaka, Uganda.
Reagan MuhweziDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Theophilus PiusDepartment of Medical Laboratory Science, Kampala International University Western Campus, Ishaka, Uganda.
Priscilla Peter DilliDepartment of Public Health, Kampala International University, Western Campus, Ishaka, Uganda.
Saheed Adekunle AkinolaDepartment of Microbiology and Parasitology, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Butare, Rwanda.
Ezera AgwuDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study presents the whole-genome sequence of Staphylococcus aureus strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate's resistance and virulence potential. DATA DESCRIPTION: Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.

Indexed as

Diabetic FootDrug Resistance, Multiple, BacterialGenome, BacterialStaphylococcus aureusWhole Genome SequencingHumansPhylogenyStaphylococcal InfectionsUgandaVirulenceComplete genome sequence of S. aureusDiabetic foot ulcerStaphylococcus aureusWhole-genome sequencingWound bacterium

Identifiers

PMID40652213
PMCPMC12256001

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.