ArticleNucleic acids research2025
Expanding genetic engineering capabilities in Vibrio natriegens with the Vnat Collection.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Metabolic engineering ofSynthetic and systems biotechnology · 2026Article
- Is faster always better? A critical assessment of Vibrio natriegens as microbial host for recombinant protein production.Bioprocess and biosystems engineering · 2026Review
- Nitrogenase regulation inbioRxiv : the preprint server for biology · 2026Article
- Marine Vibrio Biocatalysts as Unique Green Transformation (GX) Tools at the Time to Sustainable Development Goals (SDGs).Current microbiology · 2026Review
- Mitigating protocatechuic acid toxicity in Vibrio natriegens enables poly-3-hydroxybutyrate production from an aromatic carbon source.Applied microbiology and biotechnology · 2026Article
- Visual detection of collective highly toxic metals in water by a handheld whole-cell biosensing detector: From circuit to field.iScience · 2026Article
- Article
- InSillyClo, a User-Friendly Web Application to Assist Large-Scale Golden Gate Cloning and MoClo Workflows.ACS synthetic biology · 2026Article
- Genetic make-up and regulation of the L-lysine biosynthesis pathway inMicrobial cell (Graz, Austria) · 2026Article
- CloneCoordinate: Open-Source Software for Collaborative DNA Construction.ACS synthetic biology · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Vibrio natriegens, with its exceptionally fast growth rate, has great promise as a revolutionary chassis for synthetic biology, yet the realization of its full potential has been limited by the lack of robust, standardized genetic tools. Here, we present the Vnat Collection, a comprehensive, modular toolkit specifically engineered to overcome these limitations. Leveraging optimized Golden Gate cloning strategies, we introduce improved junction sequences and a highly efficient dropout part system, achieving up to a 300-fold increase in assembly efficiency. Our toolkit significantly expands the synthetic biology toolbox by providing a wide array of characterized inducible promoters, enabling precise, orthogonal gene regulation, and novel operon connectors to streamline the construction of multi-gene pathways critical for metabolic engineering. Furthermore, we enhance genome editing workflows through refined NT-CRISPR methods, incorporating homology-flanked targeting constructs and demonstrating a simplified protocol that eliminates intermediate purification steps. With over 220 rigorously validated modular components, the Vnat Collection establishes an advanced standard for genetic engineering of V. natriegens, empowering researchers to efficiently harness this organism's unparalleled potential for diverse biotechnology applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.