Evidence map›Paper›PMID 40649872›Full record

ArticleInternational journal of molecular sciences2025

Isolation and Characterization of Tissue-Derived Extracellular Vesicles from Mouse Lymph Nodes.

Bernadett R Bodnár, Sayam Ghosal, Brachyahu M Kestecher, Panna Királyhidi, András Försönits, Nóra Fekete, Edina Bugyik, Zsolt I Komlósi, Éva Pállinger, György Nagy and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Bernadett R BodnárInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.ORCID 0000-0003-3347-9225
Sayam GhosalInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.ORCID 0000-0001-6618-930X
Brachyahu M KestecherInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.ORCID 0009-0000-5689-7548
Panna KirályhidiInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
András FörsönitsInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
Nóra FeketeInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
Edina BugyikInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
Zsolt I KomlósiInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.ORCID 0000-0002-4149-1497
Éva PállingerInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.ORCID 0000-0002-5789-0951
György NagyInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
Edit I BuzásInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
Xabier OsteikoetxeaInstitute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.ORCID 0000-0003-3628-0174

Funding

European Union Horizon 2020 739593National Research, Development and Innovation Office 135637 OTKA-KNational Research, Development and Innovation Office 147023 OTKA-FKNational Research, Development and Innovation Office 150767 AdvancedNational Research, Development and Innovation Office 151417 ExcellenceNational Research, Development and Innovation Office 2019 - 2.1.7-ERA-NET-2021-00015National Research, Development and Innovation Office EKÖP-2024-237National Research, Development and Innovation Office NVKP_16-1-2016-0004National Research, Development and Innovation Office RRF-2.3.121-2022-00003National Research, Development and Innovation Office TKP2021-EGA-23National Research, Development and Innovation Office VEKOP-2.3.2-162016-00002National Research, Development and Innovation Office VEKOP-2.3.3-15-2017-00016
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are lipid membrane-enclosed particles released by all cells and can be isolated from various sources, even from solid tissues. This study focuses on isolating and characterizing EVs from mouse lymph nodes (LNs). Male C57BL/6 mice were injected with complete Freund's adjuvant, with or without ovalbumin. Inguinal and popliteal LNs were incised 9 days after immunization, and EV isolation was carried out using a combination of differential centrifugation and size-exclusion chromatography. The characteristic morphology of small and large EVs was confirmed by transmission electron microscopy. Particle size distribution and concentration were determined by nanoparticle tracking analysis, while protein and lipid contents were measured by bicinchoninic acid assay, and sulfo-phospho-vanillin assays, respectively, to calculate the protein-to-lipid ratio. Immune and EV markers were analyzed by using flow cytometry and Western blot assay, revealing significant changes between immunized mice compared to controls. This study establishes a novel protocol for isolating and characterizing EVs from LNs and highlights the impact of immunization on EV properties, offering insights into their roles in immune processes.

Indexed as

Extracellular VesiclesLymph NodesAnimalsMaleMiceMice, Inbred C57BLParticle Sizeexosomesextracellular vesiclesimmunizationlymph nodeovalbumin

Identifiers

PMID40649872
PMCPMC12249715

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.