Solid-Phase Oligosaccharide Synthesis with Highly Complexed Peptidoglycan Fragments.

Yuichiro Kadonaga, Ning Wang, Atsushi Shimoyama, Yukari Fujimoto, Koichi Fukase

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Denuded peptidoglycan oligosaccharides enable the biochemical investigation of bacterial cell wall recognition, modification, and degradation.The Journal of biological chemistry · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yuichiro KadonagaDivision of Science, Institute for Radiation Sciences, The University of Osaka, 1-1 Machikaneyama, Toyonaka 560-0043, Osaka, Japan.

Ning WangState Key Laboratory of Biopharmaceutical Preparation and Delivery, Institute of Process Engineering, University of Chinese Academy of Sciences, 1 North 2nd Street, Zhongguancun, Haidian District, Beijing 100190, China.

Atsushi ShimoyamaDepartment of Chemistry, Graduate School of Science, The University of Osaka, 1-1 Machikaneyama, Toyonaka 560-0043, Osaka, Japan.ORCID 0000-0003-1910-0450

Yukari FujimotoDepartment of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, Japan.

Koichi FukaseDivision of Science, Institute for Radiation Sciences, The University of Osaka, 1-1 Machikaneyama, Toyonaka 560-0043, Osaka, Japan.ORCID 0000-0001-8844-0710

Funding

Culture, Sports, and Technology (MEXT) of Japan Osaka University Global COE program (Frontier Biomedical Science Underlying Organelle Net-work Biology)ERATO Murata Lipid Active Structure Project; and Next Generation World-Leading Researchers NEXT Program; LR025Grant-in-Aid for Scientific Research on Innovative Areas ''Reaction Integration'' 2105Grants-in-Aid for Scientific Research 23241074Grants-in-Aid for Scientific Research JP23K06049

6 · The paper itself

Abstract

Peptidoglycan (PGN) is a component of bacterial cell walls; its fragments are recognized by the cytoplasmic receptors Nod1 and Nod2, thereby promoting the production of inflammatory cytokines and antibodies. To further elucidate these biological defense mechanisms, a large and stable supply of the PGN fragments via chemical synthesis is essential. However, the synthesis and purification of long PGN fragments are quite challenging due to their low solubility. In this study, we efficiently synthesized PGN fragments via solid-phase oligosaccharide synthesis (SPOS). Using the JandaJel™ Wang resin (JJ-Wang), an octasaccharide glycan chain of PGN was constructed by repeating glycosylation reactions to elongate β-1,4-linked disaccharide units composed of MurNAc and GlcNAc. To enhance reactivity, glycosylation was performed in a mixed solvent comprising C

Indexed as

OligosaccharidesPeptidoglycanSolid-Phase Synthesis TechniquesGlycosylationOligosaccharidesPeptidoglycanglycosylationinnate immunitypeptidoglycansolid-phase oligosaccharide synthesis

Identifiers

PMID40649302

DOI10.3390/molecules30132787

PMCPMC12251441

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.