Evidence map›Paper›PMID 40648287›Full record

ArticleSensors (Basel, Switzerland)2025

Optical Spectroscopic Detection of Mitochondrial Biomarkers (FMN and NADH) for Hypothermic Oxygenated Machine Perfusion: A Comparative Study in Different Perfusion Media.

Lorenzo Agostino Cadinu, Keyue Sun, Chunbao Jiao, Rebecca Panconesi, Sangeeta Satish, Fatma Selin Yildirim, Omer Faruk Karakaya, Chase J Wehrle, Geofia Shaina Crasta, Fernanda Walsh Fernandes and 8 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Sensors (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Lorenzo Agostino CadinuDepartment of Electric and Electronic Engineering, University of Cagliari, Via Marengo 2, 09123 Cagliari, Italy.ORCID 0009-0007-6637-2535
Keyue SunDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.ORCID 0000-0002-8928-4630
Chunbao JiaoDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.ORCID 0000-0002-9566-8243
Rebecca PanconesiDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Sangeeta SatishDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Fatma Selin YildirimDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Omer Faruk KarakayaDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.ORCID 0009-0000-4950-139X
Chase J WehrleTransplantation Center, Cleveland Clinic, Cleveland, OH 44195, USA.ORCID 0000-0002-9275-4744
Geofia Shaina CrastaDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Fernanda Walsh FernandesDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.ORCID 0000-0003-1260-7005
Nasim EshraghiDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Koki TakaseDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Hiroshi HorieDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Pier Carlo RicciDepartment of Physics, University of Cagliari, Complesso Universitario di Monserrato, S.P. Monserrato-Sestu Km 0,700, 09042 Monserrato, Italy.ORCID 0000-0001-6191-4613
Davide BagnoliMedica Spa, Via Della Beverara, 46/d, 40131 Bologna, Italy.
Mauricio Flores CarvalhoBridge to Life Ltd., Atlanta, GA 30096, USA.ORCID 0000-0001-6860-5733
Andrea SchlegelDepartment of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Massimo BarbaroDepartment of Electric and Electronic Engineering, University of Cagliari, Via Marengo 2, 09123 Cagliari, Italy.ORCID 0000-0001-6136-7664

Funding

Università degli Studi di Cagliari, Sarmed Srl 38-033-04-DOT1304377-2303
6 · The paper itself

Abstract

Ex situ machine perfusion has emerged as a pivotal technique for organ preservation and pre-transplant viability assessment, where the real-time monitoring of mitochondrial biomarkers-flavin mononucleotide (FMN) and nicotinamide adenine dinucleotide (NADH)-could significantly mitigate ischemia-reperfusion injury risks. This study develops a non-invasive optical method combining fluorescence and UV-visible spectrophotometry to quantify FMN and NADH in hypothermic oxygenated perfusion media. Calibration curves revealed linear responses for both biomarkers in absorption and fluorescence (FMN: λ

Indexed as

MitochondriaNADPerfusionAnimalsBiomarkersBiosensing TechniquesHumansOxygenSpectrometry, FluorescenceBiomarkersNADOxygenmethodologies and protocolsmitochondrial molecular biomarkersoptic phenomena in perfusion solutionoptic sensor theoretical foundationsspectroscopic calibrationspectroscopy application

Identifiers

PMID40648287
PMCPMC12251590

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.