Novel Magnetically Charged Grafts for Vascular Repair: Process Optimization, Mechanical Characterization and In Vitro Validation.

In the last decade, magnetic nanoparticles (MNPs) have attracted much attention for the implementation of non-invasive approaches suitable for the diagnosis and treatment of vascular diseases. In this work, the optimization of novel vascular grafts loaded with Nickel-based nanoparticles via electrospinning is proposed. Two different polycarbonate urethanes-i.e., Corethane A80 (COT) and Chronoflex AL80 (CHF)-were used to fabricate 3D electrospun nanocomposite grafts. SEM analysis showed a homogeneous distribution of fibers, with slight differences in terms of average diameters as a function of the polymer used-(1.14 ± 0.18) µm for COT, and (1.33 ± 0.23) µm for CHF-that tend to disappear in the presence of MNPs-(1.26 ± 0.19) µm and (1.26 ± 0.213) µm for COT/NPs and CHF/NPs, respectively. TGA analyses confirmed the higher ability of CHF to entrap MNPs in the fibers-18.25% with respect to 14.63% for COT-while DSC analyses suggested an effect of MNPs on short-range rearrangements of hard/soft micro-domains of CHF. Accordingly, mechanical tests confirmed a decay of mechanical strength in the presence of MNPs with some differences depending on the matrix-from (6.16 ± 0.33) MPa to (4.55 ± 0.2) MPa (COT), and from (3.67 ± 0.18) MPa to (2.97 ± 0.22) MPa (CNF). The in vitro response revealed that the presence of MNPs did not negatively affect cell viability after 7 days in in vitro culture, suggesting a promising use of these materials as smart vascular grafts able to support the actuation function of vessel wall muscles.