Evidence map›Paper›PMID 40646851›Full record

ArticleAnimals : an open access journal from MDPI2025

Taxonomic Profile of Cultivable Microbiota from Adult Sheep Follicular Fluid and Its Effects on In Vitro Development of Prepubertal Lamb Oocytes.

Slavcho Mrenoshki, Letizia Temerario, Antonella Mastrorocco, Grazia Visci, Elisabetta Notario, Marinella Marzano, Nicola Antonio Martino, Daniela Mrenoshki, Giovanni Michele Lacalandra, Graziano Pesole and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Slavcho MrenoshkiDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Ss. Cyril and Methodius University in Skopje, 1000 Skopje, North Macedonia.ORCID 0000-0002-0923-7229
Letizia TemerarioDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.ORCID 0000-0001-5673-6111
Antonella MastroroccoDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.ORCID 0000-0001-7136-4029
Grazia VisciDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.ORCID 0000-0003-3238-112X
Elisabetta NotarioInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche, 70126 Bari, Italy.
Marinella MarzanoInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche, 70126 Bari, Italy.ORCID 0000-0002-9996-8817
Nicola Antonio MartinoDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.ORCID 0000-0001-6853-2757
Daniela MrenoshkiDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano, Italy.ORCID 0000-0002-6400-4890
Giovanni Michele LacalandraDepartment of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano, Italy.ORCID 0000-0001-9109-3561
Graziano PesoleDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.ORCID 0000-0003-3663-0859
Maria Elena Dell'AquilaDepartment of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aims of the present study were to analyze the taxonomic profile and to evaluate the functional effects of sheep FF cultivable microbiota on prepubertal lamb oocytes PLOs developmental potential. Ovarian FFs were recovered from slaughtered adult sheep via the aspiration of developing follicles and used for microbiota propagation. Bacterial pellets underwent 16S rRNA gene sequencing and targeted culturomics, whereas cell-free supernatants were used as supplements for the in vitro maturation (IVM) of slaughtered PLOs. For the first time, bacteria presence in adult sheep FF was detected, with the first report of

Indexed as

bacterial cell-free supernatants (bCFSs)follicular fluid (FF)in vitro embryo culture (IVC)in vitro oocyte maturation (IVM)microbiotaprepubertal lamb oocytes (PLOs)sheep

Identifiers

PMID40646851
PMCPMC12249267

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.