Evidence map›Paper›PMID 40646370›Full record

ArticleMikrochimica acta2025

Dual-Check CRISPR-SERS strategy for sensitively detecting Monkeypox DNA and its single-base mutated DNA.

Yansheng Liu, Haoran Liu, Guofu Wang, Xiaobo Jia, Jin Zhou, Xinwei Dong, Hongqi Li, Haixin Chang, Zhaoxu Li, Zhicheng Jin and 1 more

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yansheng LiuSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China. Yansheng.liu@gxust.edu.cn.
Haoran LiuSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Guofu WangSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China. gfwang@guet.edu.cn.
Xiaobo JiaSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Jin ZhouSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Xinwei DongSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Hongqi LiSchool of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Haixin ChangQuantum-Nano Matter and Device Lab, State Key Laboratory of Material Processing and Die and Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan City, 430074, Hubei, China.
Zhaoxu LiHospital of Guangxi Zhuang Autonomous Region, Diecai District, No.2, Diecai Road, Guilin City, 541001, Guangxi, China.
Zhicheng JinDepartment of Chemistry, Georgia State University, Natural Science Center, 50 Decatur Street SE, Atlanta, GA, 30303, USA.
Kang LiFaculty of Computing, Engineering & Science, University of South Wales, Cardiff City, Wales, CF37 1DL, UK.

Funding

Guangxi Natural Science Foundation Project 2025GXNSFHA069082Guangxi Science and Technology Project AD22035215Key Research and Development Program of Guangxi AB22080055National Key Research and Development Program of China 2022YFE0134600
6 · The paper itself

Abstract

This study presents a convenient and efficient dual-check strategy for detecting Monkeypox DNA utilizing the SERS (Surface-enhanced Raman Spectroscopy)-CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system. The Monkeypox plasmid DNA (mpDNA) is recognized by the CRISPR RNA (crRNA)-Cas12a protein complex, where crRNA encompasses a targeting complementary sequence. Upon recognition, the trans-cleavage activity of Cas12a is activated and trans-cleaves the probe DNA (Cy3-ssDNA) which is modified on Au nanoparticles (AuNPs). As the ssDNA strand is cleaved, Cy3 molecules are released in the solution, while the amount of Cy3 modified on the AuNPs decreases. The free Cy3 molecules are collected from the supernatant, and their SERS intensities are measured using the silver nanopillar (AgNRs) substrate. The mpDNA with varying concentration from 5 nM to 0.5 fM can be quantitatively determined based on the SERS signals of free Cy3 and the collected nanotag. This strategy allows the detection of mpDNA with a concentration of 50 fM within 60 min. Moreover, the strategy can successfully detect single-base mutated mpDNA. Owing to the non-specific trans-cleavage activity of the protein, this strategy can be adapted to various nucleic acid detection scenarios by designing complementary RNA and DNA sequences.

Indexed as

CRISPR-Cas SystemsDNA, ViralSpectrum Analysis, RamanBacterial ProteinsCarbocyaninesCRISPR-Associated ProteinsDNA, Single-StrandedEndodeoxyribonucleasesGoldLimit of DetectionMetal NanoparticlesPlasmidsSilverBacterial ProteinsCarbocyaninesCas12a proteinCRISPR-Associated ProteinsDNA, Single-StrandedDNA, ViralEndodeoxyribonucleasesGoldSilverAg nanopillarsAu nanopillarsCas12aCRISPRMonkeypox DNASERS

Identifiers

PMID40646370

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.