Evidence map›Paper›PMID 40646336›Full record

ArticleFolia microbiologica2025

A novel and simple workflow for investigating Mycoplasma spp. contamination in cell cultures.

Nathalia de Freitas Michelon, José Valter Joaquim Silva Júnior, Rudi Weiblen, Eduardo Furtado Flores

Abstract read
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In one paragraph

Article in Folia microbiologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nathalia de Freitas MichelonPrograma de Pós-Graduação Em Medicina Veterinária, Universidade Federal de Santa Maria, Rio Grande Do Sul, Brazil.
José Valter Joaquim Silva JúniorDepartamento de Microbiologia E Parasitologia, Universidade Federal de Santa Maria, Rio Grande Do Sul, Brazil.
Rudi WeiblenPrograma de Pós-Graduação Em Medicina Veterinária, Universidade Federal de Santa Maria, Rio Grande Do Sul, Brazil.
Eduardo Furtado FloresPrograma de Pós-Graduação Em Medicina Veterinária, Universidade Federal de Santa Maria, Rio Grande Do Sul, Brazil. eduardofurtadoflores@gmail.com.ORCID http://orcid.org/0000-0002-5074-6025

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycoplasma spp. contamination is a major concern in laboratories handling cell cultures, and routine detection methods are usually time-consuming, laborious and lack sensitivity. This study presents a streamlined workflow integrating rapid thermal DNA extraction (99 °C

Indexed as

Cell Culture TechniquesMycoplasmaReal-Time Polymerase Chain ReactionDNA, BacterialDNA PrimersDNA, RibosomalHumansRNA, Ribosomal, 16SWorkflowDNA, BacterialDNA PrimersDNA, RibosomalRNA, Ribosomal, 16SCell cultureContaminationMycoplasma spp.Real-time PCRThermal lysis

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.