Evidence map›Paper›PMID 40645994›Full record

ArticleScientific data2025

A taxonomically reliable DNA barcode reference library for North Sea macrobenthos.

Magdalini Christodoulou, Sofie Derycke, Kevin K Beentjes, Hans Hillewaert, Silke Laakmann, Kennet Lundin, Elham Kamyab, Sahar Khodami, Sara Maes, Henning Reiss and 5 more

Abstract readDataset
In one paragraph

Article in Scientific data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. ZooKeys · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Magdalini ChristodoulouOÖ Landes-Kultur GmbH, Biodiversity Center, Linz, 4040, Austria. Magdalini.Christodoulou@ooelkg.at.ORCID 0000-0002-3597-4396
Sofie DeryckeFlanders Research Institute for Agriculture, Fisheries and Food - Marine Research (ILVO), Oostende, 8400, Belgium.
Kevin K BeentjesNaturalis Biodiversity Center, Leiden, 2333 CR, The Netherlands.
Hans HillewaertFlanders Research Institute for Agriculture, Fisheries and Food - Marine Research (ILVO), Oostende, 8400, Belgium.
Silke LaakmannHelmoltz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB), Oldenburg, 26129, Germany.
Kennet LundinGothenburg Natural History Museum, Gothenburg, S-40235, Sweden.
Elham KamyabSenckenberg am Meer, German Centre for Marine Biodiversity Research (DZMB), Wilhelmshaven, 26382, Germany.
Sahar KhodamiSenckenberg am Meer, German Centre for Marine Biodiversity Research (DZMB), Wilhelmshaven, 26382, Germany.
Sara MaesFlanders Research Institute for Agriculture, Fisheries and Food - Marine Research (ILVO), Oostende, 8400, Belgium.
Henning ReissFaculty of Biosciences and Aquaculture, Nord University, Bodø, 8026, Norway.
Carolin UhlirSenckenberg am Meer, German Centre for Marine Biodiversity Research (DZMB), Wilhelmshaven, 26382, Germany.
Laure Van den BulckeFlanders Research Institute for Agriculture, Fisheries and Food - Marine Research (ILVO), Oostende, 8400, Belgium.
Berry Van der HoornNaturalis Biodiversity Center, Leiden, 2333 CR, The Netherlands.
Annelies De BackerFlanders Research Institute for Agriculture, Fisheries and Food - Marine Research (ILVO), Oostende, 8400, Belgium.
Pedro Martinez ArbizuSenckenberg am Meer, German Centre for Marine Biodiversity Research (DZMB), Wilhelmshaven, 26382, Germany.ORCID 0000-0002-0891-1154

Funding

EC | European Regional Development Fund (Europski Fond za Regionalni Razvoj) GEANS
6 · The paper itself

Abstract

EU directives (e.g. MSFD, Habitats Directive), along with OSPAR guidelines, mandate sustainable marine resource management across national borders. Benthic organisms are crucial for assessing marine ecosystem health, but their morphological identification is time-consuming and costly. High-throughput sequencing, particularly DNA metabarcoding, offers an alternative. However, DNA-based monitoring requires substantial investment in high-quality DNA reference libraries. The GEANS project (Genetic Tools for Ecosystem Health Assessment in the North Sea Region) aimed to develop efficient DNA-based tools for benthic biomonitoring. GEANS created a curated DNA reference library (COI) for species relevant to North Sea macrobenthos monitoring, using new sequences, non-public barcode sequences, and mined sequences from GenBank and BOLD. The library, stored in a dedicated BOLD project with photographs and metadata, includes DNA barcodes for 4005 specimens from 715 species, representing over 29% of North Sea macrobenthos species. Arthropoda is the most represented, while Bryozoa and Annelida have the lowest coverage. This DNA library is expected to facilitate fast, cost-effective environmental health assessments in the North Sea for public authorities and academics.

Indexed as

Aquatic OrganismsDNA Barcoding, TaxonomicGene LibraryAnimalsEcosystemEnvironmental MonitoringNorth Sea

Identifiers

PMID40645994
PMCPMC12254315

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.