Evidence map›Paper›PMID 40645188›Full record

ArticleCell reports methods2025

Graft-seq precisely maps RNA modifications via site-specific chemical grafting strategy.

Ting Li, Ke Ding, Ziming Bao, Quan Ma, Chenyang Huang, Jie Cao, Xiao Shu, Minsong Gao, Zisheng Luo, Xushen Xiong and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ting LiMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Ke DingState Key Laboratory of Transvascular Implantation Devices, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; The Second Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 311113, China.
Ziming BaoMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Quan MaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Chenyang HuangMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Jie CaoMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China; Life Sciences Institute, Zhejiang University, Hangzhou 310058, China.
Xiao ShuMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Minsong GaoMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Zisheng LuoCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: luozisheng@zju.edu.cn.
Xushen XiongState Key Laboratory of Transvascular Implantation Devices, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; The Second Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 311113, China. Electronic address: xiongxs@zju.edu.cn.
Jianzhao LiuMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310058, China; Life Sciences Institute, Zhejiang University, Hangzhou 310058, China; State Key Laboratory of Transvascular Implantation Devices, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China; Center for RNA Medicine, International Institutes of Medicine, Zhejiang University, Yiwu 322000, China. Electronic address: liujz@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The current expansion of RNA epitranscriptomics calls for direct and high-precision mapping tools to characterize the intrinsically low abundant RNA modifications. Here, we developed a strategy, termed Graft-seq, which harnesses specific enzymatic and chemical reactions on an RNA modification site to covalently graft a known RNA branch and further utilizes the branch-to-main-chain or main-chain-to-branch landing/jumping site signal during reverse transcription to determine the locations of RNA modifications at single-base resolution. We developed a matched bioinformatics analysis pipeline for Graft-seq and successfully mapped internal N

Indexed as

RNARNA Processing, Post-TranscriptionalSequence Analysis, RNAAdenosineComputational BiologyHumansRNA, MessengerTranscriptomeAdenosineN-methyladenosineRNARNA, MessengerCP: biotechnologyCP: molecular biologydirect detection method for RNA modificationm(6)Am and NAD cap detectionnuclear RNA methylation mappingreverse transcriptase jumping in between templatesRNA m(6)A chemical grafting

Identifiers

PMID40645188
PMCPMC12296446

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.