Evidence map›Paper›PMID 40643607›Full record

ArticleArchives of microbiology2025

Integrated genomic and proteomic analysis of local Bacillus thuringiensis isolates for targeted insect pest control and functional insight.

Sumarin Soonsanga, Amporn Rungrod, Mongkon Utamatho, Chutchanun Trakulnaleamsai, Peeraphat Paenpong, Wirulda Pootakham, Narumon Phaonakrop, Sittiruk Roytrakul, Boonhiang Promdonkoy

Abstract read
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Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sumarin SoonsangaNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand. sumarin.soo@biotec.or.th.ORCID http://orcid.org/0000-0001-7342-753X
Amporn RungrodNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Mongkon UtamathoNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Chutchanun TrakulnaleamsaiNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Peeraphat PaenpongNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Wirulda PootakhamNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Narumon PhaonakropNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Sittiruk RoytrakulNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.
Boonhiang PromdonkoyNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, 12120, Pathum Thani, Thailand.

Funding

National Science and Technology Development Agency, Thailand P2351509
6 · The paper itself

Abstract

Bacillus thuringiensis (Bt) produces insecticidal crystal proteins and is widely used in pest control. Efficient strain selection for specific targets can be enhanced by integrating genomic and proteomic data. In this study, we sequenced 72 local Bt isolates and selected 12 for detailed proteomic and bioassay analyses. Expressed toxins were identified, and larval assays confirmed high toxicity in selected strains. Bt117 showed 16-fold higher toxicity against Spodoptera frugiperda compared to commercial strain B. thuringiensis serovar kurstaki, while Bt117 and Bt506 were similarly effective against Helicoverpa armigera. Comparative genomics revealed that vip3A expression is regulated by VipR, a finding confirmed experimentally. Phylogenetic analysis indicated that Bt117 and Bt202 are genomically divergent and more closely related to Bacillus cereus, suggesting horizontal gene transfer of pesticidal genes. Additionally, genes linked to plant growth-promoting traits (e.g., asbA, ipdC, and accd) were identified. This omics-guided strategy supports efficient Bt strain selection and broader application in sustainable agriculture.

Indexed as

Bacillus thuringiensisPest Control, BiologicalAnimalsBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsGenome, BacterialGenomicsHelicoverpa armigeraHemolysin ProteinsInsecticidesLarvaPhylogenyProteomicsSpodopteraBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus ThuringiensisInsecticidesVip3A protein, Bacillus thuringiensisBacillus thuringiensisCrystal proteinGenome sequencingProteomic analysisVip3AVipR

Identifiers

PMID40643607

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.