Evidence map›Paper›PMID 40643507›Full record

ArticleCells2025

Cell Counting and Cell Cycle Analysis of Simple Non-Cultured Endothelial Cell Injection (SNEC-I) Therapy: Characterization for Clinical Translation.

Darren S J Ting, Gary S L Peh, Dawn J H Neo, Xiao Yu Ng, Belinda Y L Tan, Raymond C B Wong, Hon Shing Ong, Jodhbir S Mehta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Darren S J TingTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.
Gary S L PehTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.
Dawn J H NeoTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.ORCID 0000-0003-0665-4024
Xiao Yu NgTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.
Belinda Y L TanTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.
Raymond C B WongCentre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC 3002, Australia.ORCID 0000-0002-8092-9455
Hon Shing OngTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.ORCID 0000-0001-5475-8712
Jodhbir S MehtaTissue Engineering and Cell Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.

Funding

Birmingham Health Partners NANational Medical Research Council MOH-001232-00Singapore Eye Research Institute TLF 0322-4
6 · The paper itself

Abstract

Human corneal endothelial cell therapy has recently emerged as a novel solution to treat corneal endothelial diseases. We previously demonstrated the potential of utilizing non-cultured primary corneal endothelial cells (CEnCs) isolated from donor corneas with low endothelial cell density for simple non-cultured endothelial cell injection (SNEC-I) therapy. This study aimed to develop a robust and semi-automated approach for cell counting, characterize the extent of cellular manipulation, and evaluate the translational workflow. To address this, we evaluated manual and automated cell counting approaches and characterized the extent of manipulation of CEnCs through the analysis of cell cycle status, gene expressions, and transcriptomic profiles with single-cell RNA-sequencing. The translational feasibility and functionality of SNEC-I therapy were examined using an established rabbit model of bullous keratopathy. Manual hemocytometry and automated cell-counters exhibited comparable accuracy and reproducibility. Analysis of cell cycle status, cell cycle genes (

Indexed as

Cell- and Tissue-Based TherapyCell CycleEndothelial CellsEndothelium, CornealTranslational Research, BiomedicalAnimalsCell CountDisease Models, AnimalHumansRabbitsTranscriptomebullous keratopathycell therapycorneal endothelial cellcorneal endothelial diseasecorneal transplantFuchs corneal endothelial dystrophykeratoplastyregenerative medicine

Identifiers

PMID40643507
PMCPMC12248661

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.