Evidence map›Paper›PMID 40642954›Full record

ArticleJournal of integrative plant biology2025

Development of cytosine and adenine base editors for maize precision breeding.

Xiao Fu, Nan Wang, Lina Li, Dexin Qiao, Xiantao Qi, Changlin Liu, Zhaoxu Gao, Chuanxiao Xie, Jinjie Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of integrative plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Advances and Applications of Plant Base Editing Technologies.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiao FuState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0009-0001-4042-9851
Nan WangState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0000-0001-8670-5965
Lina LiState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0009-0004-9602-015X
Dexin QiaoState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0000-0001-8842-0170
Xiantao QiState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0009-0005-1546-0258
Changlin LiuState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0000-0003-2908-8504
Zhaoxu GaoState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0000-0002-5398-8054
Chuanxiao XieState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0000-0003-3425-0211
Jinjie ZhuState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.ORCID https://orcid.org/0000-0002-9844-8043

Funding

Henan Province key research and development project 241111112300National Key Research and Development Program of China Stem Cell and Translational Research No. 2023YFD1202901Science and Technology Innovation 2030 Major projects No. 2022ZD04006Xinjiang "Leading the Charge with Open Competition" project
6 · The paper itself

Abstract

Base editing technologies can improve crops, but their efficiency in maize remains suboptimal. This study attempts to overcome these limitations by examining optimized cytosine and adenine base editors (CBEs and ABEs), namely evoAPOBEC1, evoFERNY, evoCDA1, TadA8.20, and TadA8e, for precise genome editing in transient and stable expression maize cells. Employing a seed fluorescence reporter (SFR) system for rapid screening of BE transformants and transgene-free progenies, we enhanced editing efficiencies and heritability. Notably, TadA8.20 and evoCDA1 attained multiplexed editing efficiencies of up to 100.0% and 79.0% at the tested loci, respectively, with some homozygous and bi-allelic mutants exceeding 72.4% and 73.7%. Precise editing of ZmACC1/2 (acetyl-CoA carboxylase) improved herbicide resistance, with ZmACC2 mutants displaying improved performance. This study advances crop genetic engineering by facilitating robust, multi-locus modifications without altered agronomic performance, enhancing herbicide tolerance in maize. The successful utilization of these BE is a significant step forward in agricultural biotechnology and precision breeding.

Indexed as

AdenineCytosineGene EditingPlant BreedingZea maysCRISPR-Cas SystemsMutationPlants, Genetically ModifiedAdenineCytosineadenine BEcytosine BEherbicide resistancemultiplex editingZea maysZmACC

Identifiers

PMID40642954
PMCPMC12498073

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.