Evidence map›Paper›PMID 40641647›Full record

ArticleaBIOTECH2025

An accelerated transgene-free genome editing system using microparticle bombardment of sorghum immature embryos.

Yan Zhang, Ming Cheng, Karen Massel, Ian D Godwin, Guoquan Liu

Abstract read
In one paragraph

Article in aBIOTECH, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
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  7. Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yan ZhangSchool of Agriculture and Food Sustainability, The University of Queensland, St Lucia, 4072 Australia.ORCID 0000-0003-1298-1013
Ming ChengPlant Genetic Engineering Laboratory, School of Agriculture and Food Sustainability, The University of Queensland, St Lucia, 4072 Australia.ORCID 0000-0002-2041-154X
Karen MasselCentre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, 4072 Australia.ORCID 0000-0002-9948-2438
Ian D GodwinCentre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, 4072 Australia.ORCID 0000-0002-4006-4426
Guoquan LiuCentre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, 4072 Australia.ORCID 0000-0001-5979-785X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The key factors for genome-editing in plants using CRISPR/Cas9, such as the Cas9 nuclease and guide RNA (gRNA) are typically expressed from a construct that is integrated into the plant genome. However, the presence of foreign DNA in the host genome causes genetic and regulatory concerns, particularly for commercialization. To address this issue, we developed an accelerated pipeline for generating transgene-free genome-edited sorghum ( Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-025-00204-9.

Indexed as

CRISPR/Cas9Genome editingPDS geneSorghum transformationTransgene-freeTransient gene expression

Identifiers

PMID40641647
PMCPMC12238444

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.