ReviewaBIOTECH2025
Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.
Review in aBIOTECH, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Loss of Nod25 function does not affect nitrogen-fixing symbiosis in Medicago truncatula.Plant molecular biology · 2026Article
- Engineering Symbiotic Nitrogen Fixation for Agriculture: Predominant Role of Host Plants and Fine-Tuning Regulation.Plants (Basel, Switzerland) · 2026Article
- An NSP2-MYB module orchestrates flavonoid biosynthesis and nodule symbiosis.Current biology : CB · 2026Article
- Advances in rhizobial technology: driving sustainable agriculture in the 21 st century.Archives of microbiology · 2026Review
- Integrating multi-omics approaches to shape legume root system architecture under drought stress: a comprehensive review.Frontiers in plant science · 2026Review
- Review
- Single-Cell Omics in Legumes: Research Trends and Applications.Plants (Basel, Switzerland) · 2025Review
- From Model to Crop: Roles of Macroautophagy inGenes · 2025Review
- Dissecting the Functional Interplay Between Heme Oxygenase LjHO1 and Leghemoglobins inBiology · 2025Article
- Industrial Microbial Technologies for Feed Protein Production from Non-Protein Nitrogen.Microorganisms · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The advent of genome editing technologies, particularly CRISPR/Cas9, has significantly advanced the generation of legume mutants for reverse genetic studies and understanding the mechanics of the rhizobial symbiosis. The legume-rhizobia symbiosis is crucial for sustainable agriculture, enhancing nitrogen fixation and improving soil fertility. Numerous genes with a symbiosis-specific expression have been identified, sometimes exclusively expressed in cells forming infection threads or in nitrogen-fixing nodule cells. Typically, mutations in these genes do not affect plant growth. However, in some instances, germline homozygous mutations can be lethal or result in complex pleiotropic phenotypes that are challenging to interpret. To address this issue, a rhizobia-inducible and cell-type-specific CRISPR/Cas9 strategy was developed to knock-out genes in specific legume transgenic root tissues. In this review, we discuss recent advancements in legume genome editing, highlighting the cell-type-specific CRISPR system and its crucial applications in symbiotic nitrogen fixation and beyond.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.