Evidence map›Paper›PMID 40641376›Full record

ArticleAnimal models and experimental medicine2025

Sheep femoral artery occlusion is well tolerated and does not result in ischemia.

Timothy Shiraev, Ziyu Wang, Lakshay Seth, Lisa Partel, Innes K Wise, Hugh Paterson, John O'Sullivan, Sean Lal, Anthony S Weiss, Paul Bannon and 1 more

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Article in Animal models and experimental medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Timothy ShiraevCentre for Heart Failure and Diseases of the Aorta, The Baird Institute for Applied Heart and Lung Research, Newtown, New South Wales, Australia.ORCID 0000-0002-6850-6943
Ziyu WangCharles Perkins Centre, University of Sydney, Sydney, New South Wales, Australia.
Lakshay SethCentre for Heart Failure and Diseases of the Aorta, The Baird Institute for Applied Heart and Lung Research, Newtown, New South Wales, Australia.
Lisa PartelSydney Imaging, Core Research Facilities, The University of Sydney, Sydney, New South Wales, Australia.
Innes K WiseLaboratory Animal Services, Core Research Facilities, The University of Sydney, Sydney, New South Wales, Australia.
Hugh PatersonCentral Clinical School - Surgery, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.
John O'SullivanCentre for Heart Failure and Diseases of the Aorta, The Baird Institute for Applied Heart and Lung Research, Newtown, New South Wales, Australia.
Sean LalCentre for Heart Failure and Diseases of the Aorta, The Baird Institute for Applied Heart and Lung Research, Newtown, New South Wales, Australia.
Anthony S WeissCharles Perkins Centre, University of Sydney, Sydney, New South Wales, Australia.
Paul BannonCentre for Heart Failure and Diseases of the Aorta, The Baird Institute for Applied Heart and Lung Research, Newtown, New South Wales, Australia.
Robert D HumeCentre for Heart Failure and Diseases of the Aorta, The Baird Institute for Applied Heart and Lung Research, Newtown, New South Wales, Australia.ORCID 0000-0003-3694-9333

Funding

Heart Foundation Vanguard Grant 107321The Baird Institute for applied Heart and Lung Research
6 · The paper itself

Abstract

objectiveSheep are commonly used as large animal pre-clinical models for investigating cardiovascular therapies, interventions, anatomy and physiology. Further, novel small diameter vascular grafts are frequently tested via implantation into sheep carotid arteries (CAs). This is because, unlike humans, acute occlusion of one or both sheep CAs is not associated with morbidity or mortality and thus provides safer experimental testing, with reduced ethical constraints, animal numbers and costs. However, to date there has been no evidence regarding sheep tolerance of femoral artery (FA) occlusion.

methodsIn this study, seven sheep underwent CA graft surgery, with digital subtraction angiography (DSA) of the CAs performed every 2 months via femoral access, for a total of 8 months. Four months into the study, the left FA of two sheep became inaccessible due to a suspected FA occlusion. Thus, femoral angiography was performed, followed by FA dissection, FA histology and retrospective analysis of both veterinarian animal monitoring and pain scores.

resultsFA angiography and histology confirmed complete left FA occlusion in two sheep. Retrospective animal monitoring demonstrated sheep with occluded FAs did not display increased pain scores or deleterious effects on their gait or wellbeing.

conclusionOur data shows that sheep tolerate FA occlusion with no symptoms, similar to their cerebral circulation, making them an appropriate model for assessing small diameter femoral graft interposition studies and testing other cardiovascular interventions.

Indexed as

Arterial Occlusive DiseasesFemoral ArteryIschemiaAngiography, Digital SubtractionAnimalsCarotid ArteriesDisease Models, AnimalFemaleSheepanimal modelsanimal welfare and ethicscardiovascular disordersfemoral arterygraftsocclusionsheepsmall diameter graftsvascular grafts

Identifiers

PMID40641376
PMCPMC12431566

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.