Evidence map›Paper›PMID 40641325›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Manipulating Unisexual-Sexual Reproduction Transition to Engineer Genome-Reconstructed Polyploids.

Meng Lu, Qin-Can Zhang, Zi-Yu Zhu, Yang Wang, Zhong-Wei Wang, Xi-Yin Li, Zhi Li, Xiao-Juan Zhang, Li Zhou, Jian-Fang Gui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Meng LuState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Qin-Can ZhangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zi-Yu ZhuState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Yang WangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zhong-Wei WangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Xi-Yin LiState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zhi LiState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Xiao-Juan ZhangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Li ZhouState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Jian-Fang GuiState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, The Innovation Academy of Seed Design, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.ORCID https://orcid.org/0000-0003-4969-1482

Funding

China Agriculture Research System of MOF and MARA CARS-45-07National Key Research and Development Project 2023YFD2400201National Natural Science Foundation of China 31930111National Natural Science Foundation of China 32473149Natural Science Foundation of Wuhan 2024040701010070Science and Technology Major Project in Hubei Province 2023BBA001Special funds for State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS) 2024BBSA02Special Support Funding of Chinese Academy of Sciences E529220101Strategic Priority Research Program of Chinese Academy of Sciences XDA024030104Young Elite Scientists Sponsorship Program by CAST 2023QNRC001
6 · The paper itself

Abstract

Both unisexuality and polyploidy are significant in agriculture, exhibiting revolutionary biotechnology potential, as their coupling has been demonstrated to enable the design of polyploid genomes in crops. However, their applicability to animals has remained a challenge. Herein, the first case of engineering polyploid genomes with desirable traits via unisexual-sexual reproduction transition is provided. First, a group of genome-reconstructed amphitriploids (GR-A3n) is generated involving unisexual gynogenetic Carassius gibelio, sexual C. auratus, and sexual C. cuvieri. Then, we found that the gynogenesis ability have transferred from C. gibelio to some of the GR-A3n females. This study subsequently established three GR-A3n clones with distinct herpesvirus resistance and in which differential transcriptome profiles are characterized in two main hematopoietic organs. Most genes of the hemoglobin metabolism pathway is found to exhibit high expression levels, as in C. cuvieri, which led to efficient hemoglobin biosynthesis and blood oxygen homeostasis during infection, thereby resulting in strong herpesvirus resistance. Furthermore, this study determined the resistant and susceptible haplotypes derived from chromosome 12B of C. cuvieri, which should be responsible for resistance differences between GR-A3n clones. Overall, this study establishes an approach for genetic improvement through polyploid genome design in animals.

Indexed as

Genetic EngineeringPolyploidyReproductionAnimalsFemaleGenomeherpesvirus resistancepolyploid genome designpolyploidytrait fixationunisexual reproduction

Identifiers

PMID40641325
PMCPMC12412582

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.