Evidence map›Paper›PMID 40641152›Full record

ArticleACS synthetic biology2025

PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production in

Nicholas R Robertson, Chase Lenert-Mondou, Alison C Leonard, Aida Tafrishi, Stephanie Carrera, Sangcheon Lee, Yuna Aguilar, Leonardo Sanchez Zamora, Trinity Nguyen, Jesús Beltrán and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Nicholas R RobertsonBioengineering, University of California, Riverside, Riverside, California 92521, United States.
Chase Lenert-MondouBiochemistry and Molecular Biology, University of California, Riverside, Riverside, California 92521, United States.
Alison C LeonardDepartment of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado 80305, United States.
Aida TafrishiChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.
Stephanie CarreraChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.
Sangcheon LeeChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.
Yuna AguilarBioengineering, University of California, Riverside, Riverside, California 92521, United States.
Leonardo Sanchez ZamoraChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.
Trinity NguyenChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.
Jesús BeltránBotany and Plant Science, University of California, Riverside, Riverside, California 92521, United States.
Mengwan LiChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.
Sean R CutlerBotany and Plant Science, University of California, Riverside, Riverside, California 92521, United States.
Timothy A WhiteheadDepartment of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado 80305, United States.
Ian WheeldonChemical and Environmental Engineering, University of California, Riverside, Riverside, California 92521, United States.ORCID 0000-0002-3492-7539

Funding

DARPA D24AC00011-00NSF 2128016NSF 2128287NSF 2225878
6 · The paper itself

Abstract

Monoterpenes are valued for their roles as flavors, fragrances, insecticides, and energy-dense fuels. Microorganisms provide sustainable biosynthesis routes for these important molecules, but production levels remain limited. Here, we introduce a biosensor-driven microbial engineering strategy to enhance monoterpene production, specifically targeting geraniol. Using mutagenized libraries of the PYR1 receptor─a versatile biosensor from plant ABA signaling pathways with a malleable binding pocket─we screened 24 monoterpenes and identified PYR1 variants responsive to eight, including geraniol. A low background, highly selective geraniol-sensitive PYR1 variant was expressed in the thermotolerant yeast

Indexed as

Biosensing TechniquesCRISPR-Cas SystemsFungal ProteinsKluyveromycesMonoterpenesAcyclic MonoterpenesGenome, FungalMetabolic EngineeringAcyclic MonoterpenesFungal ProteinsgeraniolMonoterpenesbiosynthesisCas9functional genetic screenGeraniolnonconventional yeast

Identifiers

PMID40641152
PMCPMC12362604

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.