Evidence map›Paper›PMID 40640830›Full record

ArticleBMC veterinary research2025

Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV.

Suzhen Yang, Yaning Sun, Yunrui Xing, Lu Fan, Yongliang Li, Yanli Shang, Shujun Chai, Yunchao Liu

Abstract read
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Suzhen Yang *Institute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. 49549173@qq.com.
Yaning Sun *Institute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Yunrui Xing *Institute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Lu FanInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Yongliang LiAnhui Divinity Biological Products Co., Ltd, Anhui, 236800, China.
Yanli ShangInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Shujun ChaiInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Yunchao LiuInstitute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. yunchaoliu2012@163.com.

Funding

Henan Provincial Scientific and Technological Research Project No. 252102110064Independent Innovation Project of Henan Academy of Agricultural Sciences No. 2025ZC153Intergovernmental international scientific and technological innovation cooperation projects of National Key Research and Development Program 2024YFE0199100
6 · The paper itself

Abstract

backgroundPseudorabies (PR) is a highly contagious disease, and it causes significant economic losses to the global swine industry. Vaccination plays an important role in the prevention and control of pseudorabies virus (PRV). To evaluate vaccine efficacy, there is a need for a quick and straight forward method to monitor PRV-induced antibody levels in practice.

resultsA time-resolved fluorescence immunochromatographic (TRFIC) strip was developed for the serological detection of PRV gB antibodies in swine. Following systematic analysis and evaluation, the assay demonstrated a high degree of correlation with established reference method. The positive and negative coincidence rates between the TRFIC strip and ELISA were 96.8% and 94.2%, respectively. Furthermore, comprehensive analytical and comparative assessments revealed that the TRFIC strip exhibited no cross-reactivity with antibodies against other porcine pathogens.

conclusionGiven its high specificity, sensitivity, and convenience, the TRFIC strip is suitable for on-site detection of PRV gB antibodies and can serve as a valuable tool for monitoring PRV immune status in animal populations.

Indexed as

Antibodies, ViralChromatography, AffinityHerpesvirus 1, SuidPseudorabiesSwine DiseasesViral Envelope ProteinsAnimalsEnzyme-Linked Immunosorbent AssaySensitivity and SpecificitySwineAntibodies, ViralViral Envelope ProteinsELISAgBPRVTime-resolved fluorescence immunochromatographic strip

Identifiers

PMID40640830
PMCPMC12247421

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.