Evidence map›Paper›PMID 40640750›Full record

ArticleBMC complementary medicine and therapies2025

Exploring and evaluating the in vitro antifungal, antibiofilm and antitoxin efficacy of Artabotrys brachypetalus Benth. against Candida spp. and its toxicity in vivo assays.

Camila de Paula Siqueira, Ralciane de Paula Menezes, Meliza Arantes Souza Bessa, Nágela Bernadelli Sousa Silva, Domingos Augusto João, Tiara da Costa Silva, Diego Godina Prado, Sérgio Antônio Lemos de Morais, Alberto de Oliveira, Francisco José Torres de Aquino and 2 more

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Article in BMC complementary medicine and therapies, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

12 authors.

Camila de Paula SiqueiraLaboratory of Antimicrobial Testing, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Ralciane de Paula MenezesLaboratory of Antimicrobial Testing, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Meliza Arantes Souza BessaLaboratory of Antimicrobial Testing, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Nágela Bernadelli Sousa SilvaLaboratory of Antimicrobial Testing, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Domingos Augusto JoãoDepartamento de Ciências Exactas E Tecnológicas, Universidade Pùngué, Chimoio, Mozambique.
Tiara da Costa SilvaInstitute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Diego Godina PradoInstitute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Sérgio Antônio Lemos de MoraisInstitute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Alberto de OliveiraInstitute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Francisco José Torres de AquinoInstitute of Chemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Luís Carlos Scalon CunhaDepartment of Chemistry, Federal Institute of the Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.
Carlos Henrique Gomes MartinsLaboratory of Antimicrobial Testing, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. carlos.martins2@ufu.br.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 307974/2019-7Fundação de Amparo à Pesquisa do Estado de Minas Gerais 12152
6 · The paper itself

Abstract

backgroundGiven the few studies found in the literature and the search for new substances with antifungal and antivirulence activities against Candida spp., the use of natural products provides us with an effective treatment of infections with minimized side effects. This study was aimed at evaluating the toxicity and antifungal activity of Artabotrys brachypetalus Benth. extract and fractions against Candida spp.

methodsThis study evaluated the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC), the ability to inhibit and/or eradicate preformed biofilm, and the ability to reduce and/or inhibit exoenzymatic production (hemolytic, phospholipase and proteinase) by Candida albicans, C. glabrata, C. krusei, C. parapsilosis and C. tropicalis against Artabotrys brachypetalus Benth. extract and fractions., The most active extract and fractions were analyzed by HPLC-ESI-MS for compound annotation. In vivo toxicity assays were performed on C. elegans larvae.

resultsHPLC-ESI-MS analysis showed mainly the presence of alkaloids in the ethanolic root extract and fractions of A. brachypetalus. The ethanolic root extract exhibited the most promising results, being fungicidal for C. glabrata with MIC/MFC of 375 µg/mL, MICB

conclusionThe remarkable A. brachypetalus ethanolic root extract exhibits minimal toxicity in C. elegans assays, coupled with its potent antifungal, antibiofilm and antivirulence capabilities against Candida spp. marking it as a standout candidate for the control of these microorganisms.

Indexed as

Antifungal AgentsBiofilmsCandidaPlant ExtractsAnimalsCaenorhabditis elegansMicrobial Sensitivity TestsAntifungal AgentsPlant ExtractsA. brachypetalusAntifungal activityAntivirulenceBiofilmCandida spp.HemolyticPhospholipaseProteinase

Identifiers

PMID40640750
PMCPMC12243214

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.