Evidence map›Paper›PMID 40639911›Full record

ArticleGenes & development2025

SUMO2 promotes histone pre-mRNA processing by stabilizing histone locus body interactions and facilitating U7 snRNP assembly.

Shuying He, Pin Lyu, Marnie W Skinner, Anthony Desotell, Brendan Foley, Chance M McCaig, Wei Wang, Jiang Qian, Liang Tong, William F Marzluff and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes & development, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shuying HeDepartment of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.ORCID 0000-0002-7478-7171
Pin LyuDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Marnie W SkinnerDepartment of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Anthony DesotellDepartment of Biological Sciences, Columbia University, New York, New York 10027, USA.
Brendan FoleyDepartment of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Chance M McCaigDepartment of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Wei WangDepartment of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
Jiang QianDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Liang TongDepartment of Biological Sciences, Columbia University, New York, New York 10027, USA.
William F MarzluffDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Michael J MatunisDepartment of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA; mmatuni1@jhu.edu.

Funding

TRAINING IN AREAS FUNDAMENTAL TO CANCER RESEARCHT32CA009110 · NCI · JOHNS HOPKINS UNIVERSITY · PI MATUNIS, MICHAEL J., WEERARATNA, ASHANI T · 1985 to 2025
$13.1M
Structural and functional studies of mRNA processing, stability and quality controlR35GM118093 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI LIANG TONG · 2016 to 2026
$8.5M
Regulation and Function of SUMO Protein Modification -Equipment SupplementR01GM060980 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI MATUNIS, MICHAEL J. · 2000 to 2023
$7.1M
NCI NIH HHS T32 CA009110NIGMS NIH HHS R01 GM060980NIGMS NIH HHS R35 GM118093
6 · The paper itself

Abstract

Histone mRNAs are the only nonpolyadenylated mRNAs in eukaryotic cells and require specialized processing in the histone locus body (HLB), a nuclear body where essential processing factors, including the U7 snRNP, are concentrated. Recent studies have revealed that misregulation of histone pre-mRNA processing can lead to polyadenylation of histone mRNAs and disruption of histone protein homeostasis. Despite links to human disease, the factors contributing to polyadenylation of histone mRNAs and the mechanisms underlying HLB assembly and U7 snRNP biogenesis remain unclear. Here, we report novel functions of the small ubiquitin-related modifier 2 (SUMO2) in promoting histone pre-mRNA processing. Using a SUMO2 knockout osteosarcoma cell line, we identified a defect in 3' end cleavage and a global increase in histone mRNA polyadenylation. Subsequent analysis of HLBs revealed increased dynamics and reduced levels of the U7 snRNP complex. By overexpressing the U7 snRNP-specific components Lsm11 and U7 snRNA, we rescued U7 snRNP levels and processing defects in SUMO2 knockout cells. Through analysis of Lsm11, we identified a SUMO-interacting motif in its N terminus required for efficient formation of U7 snRNP. Collectively, we demonstrated that SUMO2 promotes histone pre-mRNA 3' end processing by stabilizing HLB interactions and facilitating U7 snRNP assembly.

Indexed as

HistonesRibonucleoprotein, U7 Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalSmall Ubiquitin-Related Modifier ProteinsCell Line, TumorHumansPolyadenylationRNA, MessengerHistonesRibonucleoprotein, U7 Small NuclearRNA, MessengerRNA PrecursorsSmall Ubiquitin-Related Modifier ProteinsSUMO2 protein, humanhistone locus bodyhistone pre-mRNA processingSUMOU7 snRNP

Identifiers

PMID40639911
PMCPMC12305476

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.