Step-by-Step Application of Proximity-Dependent Biotinylation Tools for Identification of Astrocyte and Neuron Subproteomes In Vivo Across the Whole Brain.

Joselyn S Soto, Baljit S Khakh

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Joselyn S SotoDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. joselynsoto@fullerton.edu.

Baljit S KhakhDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neurons and astrocytes interact in the brain to form a complex network with physical, chemical, and electrical connectivity. Traditional methods for investigating proteomes have primarily employed either primary cell cultures or cells acutely separated from tissue and purified based on cell surface markers. However, these methods often compromise the cellular physiological integrity, leading to loss of associated proteins and native connectivity. Therefore, to understand the role of astrocytes and their interactions with neurons at the protein level, we designed a cell-specific method using the biotin ligase BioID2, which enables tagging of proteomes in distinct subcellular compartments of astrocytes and neurons in vivo. Here, we describe step-by-step protocols for the in vivo expression of BioID2, followed by biotinylation and subsequent purification of biotinylated proteins for mass spectrometry analysis using a brain-wide adeno-associated viral (AAV) approach.

Indexed as

AstrocytesBiotinylationBrainNeuronsProteomeProteomicsAnimalsCarbon-Nitrogen LigasesDependovirusMiceCarbon-Nitrogen LigasesProteomeAstrocytesBioID2Cell-specific proteomicsGliaIn vivo biotinylationNeuronal interactionsNeuronsProtein biology

Identifiers

PMID40638055

DOI10.1038/laban0511-155

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.