Evidence map›Paper›PMID 40638044›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Probing Pairwise Protein-Protein Interactions with the Bacterial POLAR Assay.

Coralie Tesseur, Géraldine Laloux

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Coralie Tesseurde Duve Institute, UCLouvain, Brussels, Belgium.
Géraldine Lalouxde Duve Institute, UCLouvain, Brussels, Belgium. geraldine.laloux@uclouvain.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The PopZ-linked apical recruitment (POLAR) assay is an in vivo two-hybrid assay performed in Escherichia coli, using epifluorescence microscopy to visualize the recruitment of a prey protein fusion by a bait protein fusion tethered artificially at the cell pole. Here, we present a standardized protocol of the POLAR assay, specifically designed to investigate protein-protein interactions (PPIs) in the cytoplasm. Notably, the POLAR assay has been successfully used to validate potential PPIs identified through proximity labeling, highlighting its relevance as a complementary tool within the broader context of studying protein interaction networks.

Indexed as

Bacterial ProteinsEscherichia coliEscherichia coli ProteinsProtein Interaction MappingTwo-Hybrid System TechniquesMicroscopy, FluorescenceProtein BindingProtein Interaction MapsBacterial ProteinsEscherichia coli ProteinsCell poleEpifluorescence microscopyEscherichia coliPOLAR assayPopZProtein-protein interaction (PPI)Protein recruitment dynamicsProximity labelingTwo-hybrid assay

Identifiers

PMID40638044

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.