ArticleMethods in molecular biology (Clifton, N.J.)2025
Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering in Salmonella.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Advances in Decoding Bacterial N-Terminal Proteoforms: Technologies, Challenges, and Functional Insights.Microorganisms · 2026Review
- TRAINSPOTTER: profiling nascent protein N-termini indicative of bacterial translation initiation via deformylation-assisted N-terminomics.Nucleic acids research · 2026Article
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3 authors.
Funding
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Abstract
N-terminal proteoforms (Nt-proteoforms), which arise through alternative translation initiation, exhibit variation at their N-termini that can influence subcellular localization, protein stability, and functional roles within multiprotein complexes. Advances in riboproteogenomics, particularly in ribosome profiling, have highlighted the significance of the alternative proteome in bacteria. However, Nt-proteoforms remain largely underexplored, emphasizing the need for detailed protein-protein interaction (PPI) studies to elucidate their potentially differential biological roles.Mass spectrometry-based proximity labeling techniques offer a high-throughput method for PPI screening. Here, we present a multiplexed recombineering approach that enables the genomic integration of a promiscuous biotin ligase (PBL) via double-stranded DNA (dsDNA) recombineering while simultaneously allowing the targeted mutation of specific translation initiation sites (TIS) using single-stranded DNA (ssDNA) oligos through oligo-mediated allelic replacement (OMAR). This multiplexed recombineering approach facilitates endogenous BioID tagging and the identification of Nt-proteoform-specific proxeomes.
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Registered trials
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