Evidence map›Paper›PMID 40638042›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria.

Coralie Tesseur, Ophélie Remy, Géraldine Laloux, Yoann Santin

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Coralie Tesseurde Duve Institute, UCLouvain, Brussels, Belgium.
Ophélie Remyde Duve Institute, UCLouvain, Brussels, Belgium.
Géraldine Lalouxde Duve Institute, UCLouvain, Brussels, Belgium.
Yoann Santinde Duve Institute, UCLouvain, Brussels, Belgium. yoann.santin@uclouvain.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein-protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein networks in bacteria. In this chapter, we outline a standardized protocol for implementing miniTurbo-dependent proximity labeling in bacterial models. This approach enables the unbiased identification of interacting and proximal proteins under native conditions, providing valuable insights into bacterial cell physiology.

Indexed as

BacteriaBacterial ProteinsProtein Interaction MappingProtein Interaction MapsStaining and LabelingBacterial ProteinsBacteriaBiotinylationIn vivo proximity labelingminiTurboProtein networkProtein–protein interaction (PPI)

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.