Evidence map›Paper›PMID 40637866›Full record

ArticleArchives of virology2025

Isolation and characterization of the virulent porcine epidemic diarrhea virus strain CHGS20 in China.

Jingyi Wang, Shuanghui Yin, Sumin Pan, Xiaoqing Zhang, Yupeng Fang, Tian Zhao, Li Li, Jie Zhang, Kazimierz Tarasiuk, Yongsheng Liu and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jingyi WangKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China.
Shuanghui YinLanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu, China.
Sumin PanKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China.
Xiaoqing ZhangKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China.
Yupeng FangKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China.
Tian ZhaoKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China.
Li LiShandong BaiCe test Co., LTD, 250101, Jinan, Shandong, China.
Jie ZhangKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China.
Kazimierz TarasiukVeterinary Faculty, Agriculture University Kraków, Krakow, Poland.
Yongsheng LiuKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China. liuys4031@hevttc.edu.cn.
Zygmunt PejsakVeterinary Faculty, Agriculture University Kraków, Krakow, Poland. z@pejsak.pl.
Shunli YangKey Laboratory of Preventive Veterinary Medicine of Hebei, College of Animal Science and Technology, Hebei Normal University of Science and Technology, HNUST, 066004, Qinhuangdao, Hebei, China. ysl4181@hevttc.edu.cn.ORCID http://orcid.org/0000-0001-5937-0004

Funding

Hebei Normal University of Science and Technology 2023YB002State Scientific-Research Institute of Physiology and Basic Medicine 2024JK011
6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) is the etiological agent of porcine epidemic diarrhea (PED), which threatens the swine industry worldwide. Although commercial PEDV vaccines are available, the disease continues to plague the swine industry in China. Therefore, isolating PEDV from clinical samples is beneficial for conducting molecular epidemiological investigations, developing effective vaccines and diagnostic methods, and investigating pathogenic mechanisms. In this study, a genotype II PEDV strain, CHGS20, was isolated from a small-intestine sample collected in 2020 from a PEDV-infected piglet and propagated in Vero cells. Virus production in cell culture was confirmed by cytopathology, immunofluorescence assay, and transmission electron microscopy. The infectious virus titers during the first 10 passages ranged from 10

Indexed as

Coronavirus InfectionsPorcine epidemic diarrhea virusSwine DiseasesAnimalsAntibodies, NeutralizingAntibodies, ViralChinaChlorocebus aethiopsCytopathogenic Effect, ViralGenotypeMiceMice, Inbred BALB CPhylogenySwineVero CellsVirulenceAntibodies, NeutralizingAntibodies, Viralelectric cell-substrate impedance sensingisolationphylogenetic analysisporcine epidemic diarrhea virus

Identifiers

PMID40637866

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.