ArticleAnalytical and bioanalytical chemistry2025
Pentameric nanobodies serve as a capture agent and RANbodies function as immunoprobes for the sensitive detection of Salmonella typhimurium in immunoassays.
Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Antigen-binding-enhanced divalent nanobody-based immunomagnetic separation coupled with ATP bioluminescence for viable Salmonella detection.Analytical and bioanalytical chemistry · 2026Article
- Conformation-sensitive nanobody immunoassay enables differentiation of live and heat-inactivated Salmonella Typhimurium for food safety monitoring.Food chemistry · 2026Article
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Authors and funding
9 authors.
Funding
Abstract
A simpler, more convenient, and faster diagnostic method for detection of Salmonella typhimurium (S. typhimurium) is urgently needed. In this study, the pentameric nanobodies (pST-Nb4) and HRP fusion nanobodies (ST-Nb1-vHRP) were produced in vitro, and pST-Nb4 displayed a binding affinity to S. typhimurium that was 61.8 times higher than that of the monomeric ST-Nb4. Ultimately, the sandwich ELISA based on pST-Nb4 as a capture agent and ST-Nb1-vHRP as an immunoprobe was developed for rapid detection of S. typhimurium. The assay was validated to have good specificity with a lower detection limit of 2.37 × 10
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