ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Half-Pipe Melt Electrowritten Scaffolds Support Engineering of an Immunocompetent Hydrogel-Embedded Intestine-on-a-Chip.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Microfluidic engineering of immune-competent organs-on-chips and their applications.Frontiers in immunology · 2026Review
- Half-Pipe Melt Electrowritten Scaffolds Support Engineering of an Immunocompetent Hydrogel-Embedded Intestine-on-a-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
In vitro models that mimic intestinal mucosal tissue inflammation and assess the sensitizing capacity of food proteins are essential for understanding food allergy mechanisms and improving safety assessments. Current 2D models lack spatial epithelial-immune cell interactions, including dendritic cell (DC) migration and DC-T cell crosstalk. Intestine-on-a-chip (IoC) models are used for infections and inflammatory bowel disease (IBD) but are not yet widely used in food allergy research. Here, a 3D immunocompetent IoC model is presented using extrusion-based bioprinting and melt electrowriting (MEW). The system integrates human intestinal epithelial cells (Caco-2) seeded on half-pipe-shaped MEW scaffolds and co-cultured with hydrogel-embedded monocyte-derived DCs (moDCs) inside the printed device. Subsequent moDC-T cell interactions are studied separately in a hydrogel-embedded system. IoCs exhibited leak-tight epithelial barriers comparable to transwell-like systems, while demonstrating higher metabolic and brush border enzyme activity, and lower LDH leakage. After food allergens (peanut, milk, and egg), non-allergen (Rubisco) or pro-inflammatory stimuli (toxin A and LPS) exposure, distinguishable effects on epithelial barrier integrity and moDC driven Th1/Th2 immune responses are observed. The IoC model presents a significant step toward 3D in vitro systems that mimic the intestinal mucosa's compartments to study food allergen sensitization and inflammatory diseases.
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Registered trials
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