ArticleFrontiers in microbiology2025
Combination of fluorescent reagents with 2-(4-aminophenyl) benzothiazole and safranin O was useful for analysis of spore structure, indicating the diversity of
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Purification and Thioflavin T Staining of Bacillus spp. Endospores for Fluorescence-Based Assays.Current protocols · 2026Article
- Identification of SpoVID as a putative new substrate of the sporulation-specific protease YabG inFrontiers in microbiology · 2026Article
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3 authors.
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Abstract
Background: Safranin O is commonly used for the gram staining of bacteria and fluorescent staining of plant tissues. We aimed to perform a more detailed structural analysis of bacterial spores by analyzing the staining pattern of safranin O, together with a combination of other fluorescence probes, including 2-(4-aminophenyl) benzothiazole (APBT). Methods: We stained spores from six Results: Fluorescence microscopy analysis revealed that safranin O exhibits two distinct fluorescence peaks, green and red, in Conclusion: These findings show that safranin O produces distinct red and green fluorescence patterns in bacterial spores. The combined use of safranin O and other fluorescent probes with fluorescence microscopy and GFP fusion proteins offers a powerful approach for visualizing and analyzing bacterial spore structures. The present study on
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