Evidence map›Paper›PMID 40635039›Full record

ArticlePlant methods2025

Stomata morphology measurement with interactive machine learning: accuracy, speed, and biological relevance?

Tomke S Wacker, Abraham G Smith, Signe M Jensen, Theresa Pflüger, Viktor G Hertz, Eva Rosenqvist, Fulai Liu, Dorte B Dresbøll

Abstract read
In one paragraph

Article in Plant methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tomke S WackerDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark. tsw@plen.ku.dk.
Abraham G SmithDepartment of Computer Science, University of Copenhagen, Copenhagen, Denmark.
Signe M JensenDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Theresa PflügerDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Viktor G HertzDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Eva RosenqvistDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Fulai LiuDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
Dorte B DresbøllDepartment of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.

Funding

Danmarks Frie Forskningsfond 0217-00084BGrønt Udviklings- og Demonstrations Program 34009-20-1761Novo Nordisk Fonden NNF22OC0080177
6 · The paper itself

Abstract

Stomatal morphology plays a critical role in regulating plant gas exchange influencing water use efficiency and ecological adaptability. While traditional methods for analyzing stomatal traits rely on labor-intensive manual measurements, machine learning (ML) tools offer a promising alternative. In this study, we evaluate the suitability of a U-Net-based interactive ML software with corrective annotation for stomatal morphology phenotyping. The approach enables non-ML experts to efficiently segment stomatal structures across diverse datasets, including images from different plant species, magnifications, and imprint methods. We trained a single model based on images from five datasets and tested its performance on unseen data, achieving high accuracy for stomatal density (R

Indexed as

Model trainingModel validationRootPainterStatistical powerStomatal densityStomatal size

Identifiers

PMID40635039
PMCPMC12243431

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.