RNA-seq of penile squamous cell carcinoma shows ODC1 overexpression is associated with worse overall survival.

Brian A Keller, Gareth Palidwor, Elena Pastukhova, Zuzanna Gorski, Nicola Schieda, Kevin Hogan, Paul Borowy-Borowski, Harman Sekhon, Trevor A Flood

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Article in Virchows Archiv : an international journal of pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Brian A KellerLondon Health Sciences Centre, Western University, London, ON, Canada.

Gareth PalidworOttawa Hospital Research Institute, Bioinformatics Core Facility, Ottawa, Canada.

Elena PastukhovaDepartment of Medicine, University of Toronto, Toronto, Canada.

Zuzanna GorskiDivision of Anatomic Pathology, Dalhousie University, Halifax, Canada.

Nicola SchiedaDepartment of Radiology, The Ottawa Hospital, University of Ottawa, Ottawa, Canada.

Kevin HoganDivision of Anatomic Pathology, The Ottawa HospitalUniversity of Ottawa, 501 Smyth Road, 4 Floor CCW, Room 4259, Ottawa, ON, K1H 8L6, Canada.

Paul Borowy-BorowskiDivision of Anatomic Pathology, The Ottawa HospitalUniversity of Ottawa, 501 Smyth Road, 4 Floor CCW, Room 4259, Ottawa, ON, K1H 8L6, Canada.

Harman SekhonDivision of Anatomic Pathology, The Ottawa HospitalUniversity of Ottawa, 501 Smyth Road, 4 Floor CCW, Room 4259, Ottawa, ON, K1H 8L6, Canada.

Trevor A FloodDivision of Anatomic Pathology, The Ottawa HospitalUniversity of Ottawa, 501 Smyth Road, 4 Floor CCW, Room 4259, Ottawa, ON, K1H 8L6, Canada. tflood@toh.on.ca.ORCID http://orcid.org/0000-0002-5227-2708

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Penile squamous cell carcinoma (PSCC) is classified into human papillomavirus (HPV)-associated and HPV-independent subtypes. Aggressive and metastatic PSCCs pose significant treatment challenges due to limited effective systemic options. Polyamines are essential intracellular regulators involved in cell proliferation and tumor progression, with ODC1 serving as the rate-limiting enzyme in polyamine metabolism. We used RNA sequencing on 21 PSCC tumors to investigate ODC1 expression. ODC1 overexpression was identified in 10/21 (47.6%) tumors with a higher prevalence in HPV-independent cases (7/12, 58.3%) compared to HPV-associated tumors (3/9, 33.3%). Immunohistochemistry confirmed ODC1 positivity in 5/21 (23.8%) tumors, all of which overexpressed ODC1 and were HPV-independent. Importantly, ODC1 overexpression and positive immunostaining correlated with significantly worse overall survival (p < 0.05). These results indicate that ODC1 overexpression and positive immunostaining are associated with poor prognosis in PSCC, supporting ODC1's value as a prognostic biomarker and highlighting its potential as a novel therapeutic target, given available anti-cancer drugs targeting ODC1 activity.

Indexed as

Biomarkers, TumorCarcinoma, Squamous CellPenile NeoplasmsAdultAgedAged, 80 and overHumansImmunohistochemistryMaleMiddle AgedPapillomavirus InfectionsPrognosisRNA-SeqBiomarkers, TumorHuman papilloma virusesOrnithine decarboxylaseOrnithine decarboxylase inhibitorsPenile cancerPenisPolyaminesRNA sequence analysisSquamous cell carcinoma

Identifiers

PMID40632310

DOI10.1007/s00428-025-04166-7

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