Evidence map›Paper›PMID 40631273›Full record

ArticlebioRxiv : the preprint server for biology2025

A global genetic interaction map of a human cell reveals conserved principles of genetic networks.

Maximilian Billmann, Michael Costanzo, Xiang Zhang, Arshia Z Hassan, Mahfuzur Rahman, Kevin R Brown, Katherine S Chan, Amy Hin Yan Tong, Carles Pons, Henry N Ward and 75 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

85 authors.

Maximilian BillmannDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.ORCID 0000-0002-6556-9594
Michael CostanzoDonnelly Centre, University of Toronto; Toronto ON, Canada.ORCID 0000-0002-7906-2604
Xiang ZhangDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Arshia Z HassanDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Mahfuzur RahmanDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Kevin R BrownProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Katherine S ChanProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Amy Hin Yan TongDonnelly Centre, University of Toronto; Toronto ON, Canada.
Carles PonsInstitute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute for Science and Technology; Barcelona, Spain.ORCID 0000-0002-9131-9089
Henry N WardDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Catherine RossDonnelly Centre, University of Toronto; Toronto ON, Canada.
Jolanda van LeeuwenDonnelly Centre, University of Toronto; Toronto ON, Canada.
Michael AreggerDonnelly Centre, University of Toronto; Toronto ON, Canada.
Keith A LawsonDonnelly Centre, University of Toronto; Toronto ON, Canada.
Barbara MairDonnelly Centre, University of Toronto; Toronto ON, Canada.ORCID 0000-0001-6982-2529
Amy F RothDepartment of Pharmacology, Wayne State University School of Medicine; Detroit MI, USA.
Nesli E SenDepartment of Molecular and Cellular Biology, University of Geneva; Geneva, Switzerland.
Duncan ForsterDonnelly Centre, University of Toronto; Toronto ON, Canada.
Guihong TanDonnelly Centre, University of Toronto; Toronto ON, Canada.
Patricia MeroProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Sanna N MasudDonnelly Centre, University of Toronto; Toronto ON, Canada.
Yoonkyu LeeDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Magali Aguilera-UribeProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Matej UsajDonnelly Centre, University of Toronto; Toronto ON, Canada.
Sylvia M T AlmeidaDonnelly Centre, University of Toronto; Toronto ON, Canada.
Kamaldeep AulakhProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Urvi BhojooDonnelly Centre, University of Toronto; Toronto ON, Canada.
Saba BirkadzeProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Nathaniel BudijonoDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Xunhui CaiInstitute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Wuhan, China.
Joseph J CaumannsDonnelly Centre, University of Toronto; Toronto ON, Canada.
Megha ChandrashekharDonnelly Centre, University of Toronto; Toronto ON, Canada.
Daniel ChangDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Ryan ClimieDonnelly Centre, University of Toronto; Toronto ON, Canada.
Kuheli DasguptaProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Adrian DrazicDonnelly Centre, University of Toronto; Toronto ON, Canada.
Jose I Rojas EcheniqueDonnelly Centre, University of Toronto; Toronto ON, Canada.
Rafael GacesaDonnelly Centre, University of Toronto; Toronto ON, Canada.
Adrian Granda FariasProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Andrea HabsidProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Ira HoreckaDonnelly Centre, University of Toronto; Toronto ON, Canada.
Kristin KantautasDonnelly Centre, University of Toronto; Toronto ON, Canada.
Fenghu JiInstitute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Wuhan, China.
Dae-Kyum KimDonnelly Centre, University of Toronto; Toronto ON, Canada.
Seon Yong LeeProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Wendy LiangDonnelly Centre, University of Toronto; Toronto ON, Canada.
Julianne LimDonnelly Centre, University of Toronto; Toronto ON, Canada.
Kevin LinDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Xueibing LuDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Babak NamiProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Allison NixonDonnelly Centre, University of Toronto; Toronto ON, Canada.
Nicholas MikolajewiczProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Lyudmila NedyalkovaDonnelly Centre, University of Toronto; Toronto ON, Canada.
Thomas RohdeInstitute of Human Genetics, School of Medicine and University Hospital Bonn, University of Bonn; Bonn, Germany.
Maria Sartori RodriguesDonnelly Centre, University of Toronto; Toronto ON, Canada.
Martin SosteDonnelly Centre, University of Toronto; Toronto ON, Canada.
Eric SchultzDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Wen WangDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Ashwin SeetharamanDonnelly Centre, University of Toronto; Toronto ON, Canada.
Emira ShuteriqiDonnelly Centre, University of Toronto; Toronto ON, Canada.
Olga SizovaProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
David Thomson TaylorProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Maria TereshchenkoDepartment of Biochemistry, University of Toronto; Toronto ON, Canada.
David TieuDonnelly Centre, University of Toronto; Toronto ON, Canada.
Jacob TurowecDonnelly Centre, University of Toronto; Toronto ON, Canada.
Tajinder UbhiDonnelly Centre, University of Toronto; Toronto ON, Canada.
Sylvia VarlandDonnelly Centre, University of Toronto; Toronto ON, Canada.
Kyle E WangDonnelly Centre, University of Toronto; Toronto ON, Canada.
Zi Yang WangDonnelly Centre, University of Toronto; Toronto ON, Canada.
Jiarun WeiProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Yu-Xi XiaoProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.
Grant W BrownDonnelly Centre, University of Toronto; Toronto ON, Canada.ORCID 0000-0002-9002-5003
Benjamin F CravattDepartment of Chemistry, The Scripps Research Institute; La Jolla, CA, USA.
Scott J DixonDepartment of Biology, Stanford University; Stanford CA, USA.ORCID 0000-0001-6230-8199
Haley D M WyattDepartment of Biochemistry, University of Toronto; Toronto ON, Canada.
Hannes L RöstDonnelly Centre, University of Toronto; Toronto ON, Canada.
Frederick P RothDonnelly Centre, University of Toronto; Toronto ON, Canada.ORCID 0000-0002-6628-649X
Tian XiaSchool of Software engineering, Huazhong University of Science and Technology; Wuhan, China.
Gary D BaderDonnelly Centre, University of Toronto; Toronto ON, Canada.ORCID 0000-0003-0185-8861
Robbie LoewithDepartment of Molecular and Cellular Biology, University of Geneva; Geneva, Switzerland.
Nicholas G DavisDepartment of Pharmacology, Wayne State University School of Medicine; Detroit MI, USA.
Brenda AndrewsDonnelly Centre, University of Toronto; Toronto ON, Canada.
Chad L MyersDepartment of Computer Science and Engineering, University of Minnesota; Minneapolis, MN, USA.
Jason MoffatProgram in Genetics and Genome Biology, The Hospital for Sick Children; Toronto, ON, Canada.ORCID 0000-0002-5663-8586
Charles BooneDonnelly Centre, University of Toronto; Toronto ON, Canada.

Funding

TRAINING FOR FUTURE BIOTECHNOLOGY DEVELOPMENTT32GM008347 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI SCHMIDT-DANNERT, CLAUDIA · 1990 to 2021
$11.0M
Systematically mapping variant effects for cardiovascular genesR01HL164675 · NHLBI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ASHLEY, EUAN A, RODEN, DAN M · 2022 to 2025
$8.1M
Methods for large-scale analysis of chemical-genetic interactionsR01HG005084 · NHGRI · UNIVERSITY OF MINNESOTA · PI MYERS, CHAD L · 2010 to 2022
$2.9M
Targeted Chemical-Genetic Screen Platform for Identifying Novel AML TherapeuticsF30CA257227 · NCI · UNIVERSITY OF MINNESOTA · PI LIN, KEVIN JENG-YEN · 2021 to 2023
$141k
NCI NIH HHS F30 CA257227NHGRI NIH HHS R01 HG005084NHLBI NIH HHS R01 HL164675NIGMS NIH HHS T32 GM008347
6 · The paper itself

Abstract

We generated a genome-scale, genetic interaction network from the analysis of more than 4 million double mutants in the haploid human cell line, HAP1. The network maps ~90,000 genetic interactions, including thousands of extreme synthetic lethal and genetic suppression interactions. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes, pathways, biological processes, and cellular compartments. Comparative analyses showed that general principles of genetic networks are conserved from yeast to human cells. A genetic interaction network mapped in a single genetic background complements the DepMap gene co-essentiality network, recapitulating many of the same biological connections and also capturing unique functional information to reveal roles of uncharacterized genes and molecular determinants of specific cancer cell line genetic dependencies.

Identifiers

PMID40631273
PMCPMC12236771

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.