Evidence map›Paper›PMID 40631176›Full record

ArticlebioRxiv : the preprint server for biology2025

Ligand-dependent G protein dynamics underlying opioid signaling efficacy.

Jonathan Deutsch, Daniel Hilger, John Janetzko, Charles M Schroeder, Steven Chu, Brian K Kobilka, Rabindra V Shivnaraine

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jonathan DeutschDepartment of Biophysics and Quant Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Daniel HilgerDepartment of Pharmaceutical Chemistry, Philipps-University Marburg, Marburg, Germany.
John JanetzkoDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Charles M SchroederDepartment of Biophysics and Quant Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Steven ChuDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Brian K KobilkaDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.
Rabindra V ShivnaraineDepartment of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA.

Funding

Extending the temporal and spatial capabilities of single-molecule methodsR01GM143554 · NIGMS · STANFORD UNIVERSITY · PI CHU, STEVEN · 2021 to 2024
$2.1M
G protein coupled receptor structure, dynamics, and signaling.R35NS137408 · NINDS · STANFORD UNIVERSITY · PI Brian K Kobilka · 2024 to 2026
$1.9M
The structural basis for pathway-selective signaling by the µ opioid receptorR01DA036246 · NIDA · STANFORD UNIVERSITY · PI Brian K Kobilka · 2024 to 2026
$1.6M
Investigating the molecular details of assembly, disassembly and trafficking of GPCR-arrestin complexesK99GM147609 · NIGMS · STANFORD UNIVERSITY · PI JANETZKO, JOHN · 2022 to 2023
$225k
NIDA NIH HHS R01 DA036246NIGMS NIH HHS K99 GM147609NIGMS NIH HHS R01 GM143554NINDS NIH HHS R35 NS137408
6 · The paper itself

Abstract

Activation of heterotrimeric G proteins by G protein-coupled receptors (GPCRs) requires large-scale opening of the Gα α-helical domain (AHD) to expose the nucleotide-binding site and facilitate GDP-GTP exchange. While orthosteric ligands are known to modulate GPCR conformation and signaling efficacy, how these effects propagate to the G protein itself remains unclear. Using single-molecule fluorescence resonance energy transfer (smFRET) imaging, we monitored AHD motions in G

Identifiers

PMID40631176
PMCPMC12236782

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.