Evidence map›Paper›PMID 40631087›Full record

ArticlebioRxiv : the preprint server for biology2025

Direct from the Seed: An Atomic-Resolution Protein Structure by Ab Initio MicroED.

Purna Chandra Rao Vasireddy, Timothy Low-Beer, Katherine A Spoth, Devrim Acehan, Matthew R Crawley, Michael W Martynowycz

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Purna Chandra Rao VasireddyDepartment of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14203.
Timothy Low-BeerDepartment of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14203.
Katherine A SpothUB Hauptman-Woodward Institute, University at Buffalo, The State University of New York, Buffalo, NY 14203.
Devrim AcehanDepartment of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14203.
Matthew R CrawleyDepartment of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260.
Michael W MartynowyczDepartment of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14203.ORCID 0000-0003-0055-230X

Funding

National HTX Center: Enabling Access to State-of-the-Art Crystallization CapabilitiesR24GM141256 · NIGMS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Sarah Elizabeth Johnson Bowman · 2021 to 2026
$6.3M
NIGMS NIH HHS R24 GM141256
6 · The paper itself

Abstract

While purifying the seed protein crambin, we discovered that needles of pure protein nanocrystals formed spontaneously during the drying of a simple ethanolic purification drop. Contrary to traditional crystallography, these needles diffracted poorly using X-rays yet proved to be exceptionally well-suited for microcrystal electron diffraction (MicroED). By merging data from 58 such nanocrystals, we obtained diffraction to 0.85 Å resolution with an overall correlation coefficient of over 99% and solved the structure

Indexed as

Atomic ResolutionCryo-EMMicrocrystal electron diffractionNatural ProductPhasing

Identifiers

PMID40631087
PMCPMC12236495

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.