Evidence map›Paper›PMID 40630523›Full record

ArticleResearch square2025

High connectivity and low differentiation of Plasmodium falciparum parasite populations in a setting with high seasonal migration.

Endashaw Esayas, William Louie, Isobel Routledge, Maxwell Murphy, Nigatu Negash Demeke, Faith De Amaral, Andrés Aranda-Díaz, Bryan Greenhouse, Fikregabrail Aberra Kassa, Tedros Nigusse and 8 more

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In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Endashaw EsayasArmauer Hansen Research Institute.
William LouieUniversity of California, San Francisco.
Isobel RoutledgeUniversity of California, San Francisco.
Maxwell MurphyUniversity of California, San Francisco.
Nigatu Negash DemekeArmauer Hansen Research Institute.
Faith De AmaralUniversity of California, San Francisco.
Andrés Aranda-DíazUniversity of California, San Francisco.
Bryan GreenhouseUniversity of California, San Francisco.
Fikregabrail Aberra KassaArmauer Hansen Research Institute.
Tedros NigusseArmauer Hansen Research Institute.
Muluken AssefaArmauer Hansen Research Institute.
Temesgen AshineArmauer Hansen Research Institute.
Asefaw GetachewProgram for Appropriate Technology in Health.
Henry NtukuProgram for Appropriate Technology in Health.
Lemu GolassaAddis Ababa University.
Endalamaw GadisaArmauer Hansen Research Institute.
Adam BennettProgram for Appropriate Technology in Health.
Jennifer L SmithUniversity of California, San Francisco.

Funding

Mentoring translational scientists in international infectious disease researchK24AI144048 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Bryan R Greenhouse · 2019 to 2026
$1.6M
Genetic and social network analysis to target interventions for malaria eliminationK01AI153555 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SMITH, JENNIFER LINNEA · 2020 to 2024
$725k
Gates Foundation INV-003212NIAID NIH HHS K01 AI153555NIAID NIH HHS K24 AI144048
6 · The paper itself

Abstract

Seasonal movement of less-immune people from low- to high- transmission regions increases malaria risk and may introduce parasite strains to both areas. This study examined Plasmodium falciparum genetic diversity and connectivity between low-transmission highlands and endemic lowlands in Ethiopia to assess the contribution of seasonal agricultural migration in sustaining transmission. P. falciparum qPCR-positive dried blood spots collected from highland health facilities and lowland agricultural worksites were sequenced using multiplexed amplicon sequencing. Complexity of infection (COI) and infection pairwise relatedness were estimated and used for clustering analysis. Lowland populations (seasonal workers and local residents) had higher COI and polyclonal infection rates (mean COI 2.62, 60%, n=581) than highland residents (mean COI 2.00, 42%, n=599). Similar expected heterozygosity (He ≈0.4) was observed, and P. falciparum infections from worksites showed high genetic connectivity between highland and lowland populations, with extensive parasite sharing, including 27 identical clusters in highland cases and 12 in seasonal workers. Integrating parasite genomic data with epidemiological information revealed strong connectivity and low genetic differentiation between these regions linked by seasonal migration. These findings highlight how agricultural mobility likely drives parasite diversity and gene flow, implicating its role in sustaining malaria transmission.

Indexed as

Amplicon sequencingComplexity of infectionEthiopiaGenetic connectivityHighland-lowland populationsMalariaPlasmodium falciparumSeasonal agricultural workers

Identifiers

PMID40630523
PMCPMC12236906

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.